Team:UNIK Copenhagen/Results

From 2013.igem.org

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<h1>Results</h1>
<h1>Results</h1>
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<p>
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In our project we have successfully grown strains <i>Magnetospirrilum magnetotacticum</i> (MS-1) and <i>Magnetospirrilum gryphiswaldense</i> (MSR-1).  
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Over the summer Team Magneto worked hard and some of the preset goals were successfully accomplished. Here we show and explain you our main results.  
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<br><br>
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</p>
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We have successfully enriched magnetic bacteria in our local environment (see <a href="https://2013.igem.org/Team:UNIK_Copenhagen/TheCphStrain" target="_blank">the CPH strain</a>).
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We have created fusion protein of enhanced green fluorescent protein (eGFP) and MamC from
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<h3>Quick Overview</h3>
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<ul>
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<li><p>MamC-eGFP: the functionality of the eGFP part of the fusion was proven, regarding the MamC part only the expected size of the protein could be shown</p></li>
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<li><p>MamC alone couldn’t be further characterized</p></li>
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<li><p>eGFP: the functionality and autenticity of the protein could be shown</p></li>
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<li><p>eFbFP: the submitted BioBrick couldn’t be successfully characterized</p></li>
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</ul>
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<h3>MamC-eGFP</h3>
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<p>
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MamC (BBa_K1094001) from Magnetospirillum magnetotacticum (MS-1) was tagged with enhanced green fluorescent protein (eGFP, BBa_K1094400). Between the parts a glycine linker (10 glycine residues) is added. The gene product can be used to detect localization of MamC in MS-1.
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The two parts were cloned together using classical cloning into pJAM1786 in E. coli. Colony PCR and gel electrophoresis was performed to ensure the insert was in the vector (Fig.1). </p>

Revision as of 20:43, 3 October 2013

Results

Over the summer Team Magneto worked hard and some of the preset goals were successfully accomplished. Here we show and explain you our main results.

Quick Overview

  • MamC-eGFP: the functionality of the eGFP part of the fusion was proven, regarding the MamC part only the expected size of the protein could be shown

  • MamC alone couldn’t be further characterized

  • eGFP: the functionality and autenticity of the protein could be shown

  • eFbFP: the submitted BioBrick couldn’t be successfully characterized

MamC-eGFP

MamC (BBa_K1094001) from Magnetospirillum magnetotacticum (MS-1) was tagged with enhanced green fluorescent protein (eGFP, BBa_K1094400). Between the parts a glycine linker (10 glycine residues) is added. The gene product can be used to detect localization of MamC in MS-1. The two parts were cloned together using classical cloning into pJAM1786 in E. coli. Colony PCR and gel electrophoresis was performed to ensure the insert was in the vector (Fig.1).

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