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Team:Tuebingen/Notebook/Protocols/glycerol-stocks
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| - | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: | + | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> |
| + | <p> </p> | ||
| + | |||
| + | <h3>Procedure</h3> | ||
| + | |||
| + | <ol> | ||
| + | <li>Inoculate 5 mL <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/plates">LB-medium</a> with one single colony from a fresh plate (e.g. after <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/trafo">transformation</a>) and incubate at 37 °C and 220 rpm over night.</li> | ||
| + | <li>On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.</li> | ||
| + | <li>Add 1 mL of cell-culture and mix.</li> | ||
| + | </ol> | ||
</div> | </div> | ||
Revision as of 00:31, 4 October 2013
Glycerol Stocks
Back to Protocols
Procedure
- Inoculate 5 mL LB-medium with one single colony from a fresh plate (e.g. after transformation) and incubate at 37 °C and 220 rpm over night.
- On the next day, put 0.5 mL autoclaved glycerol (50 %) in an autoclaved 2 mL Eppendorf-tube.
- Add 1 mL of cell-culture and mix.
