Team:Tuebingen/Notebook/Protocols/gelextraction

From 2013.igem.org

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Line 26: Line 26:
   <li>Elute in 20 µL Genaxxon Buffer 3.2.</li>
   <li>Elute in 20 µL Genaxxon Buffer 3.2.</li>
   <li>Run eluate twice through the column in order to increase DNA yields.</li>
   <li>Run eluate twice through the column in order to increase DNA yields.</li>
 +
  <li>Store at -20 °C.</li>
   <li>Control success of gelextraction via <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">gelelectrophoresis</a>.</li>
   <li>Control success of gelextraction via <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols/gelelectrophoresis">gelelectrophoresis</a>.</li>
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Revision as of 02:28, 4 October 2013

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Gel Extraction
Back to Protocols

 

Procedure

  1. Follow Genaxxon PCR and Gel extraction Mini Prep Kit Manual
  2. Elute in 20 µL Genaxxon Buffer 3.2.
  3. Run eluate twice through the column in order to increase DNA yields.
  4. Store at -20 °C.
  5. Control success of gelextraction via gelelectrophoresis.


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