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Team:Tuebingen/Notebook/Protocols/tae
From 2013.igem.org
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Revision as of 12:01, 4 October 2013
TAE Buffer
Reagents
| 242.0 g | Tris Base |
| 57.1 mL | Glacial acetic acid |
| 100 mL | EDTA (c = 0.5 M ; pH 8.0) |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua bidest. Store at room temperature.
Procedure yields 50x concentration.
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