Team:Tuebingen/Notebook/Protocols/onestep

From 2013.igem.org

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SOB Medium
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ONE-STEP Buffer
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Revision as of 03:42, 4 October 2013

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ONE-STEP Buffer
Back to Protocols

 

Procedure

Dissolve 0.2 M lithium acetate and polyethylene glycol 3350 (PEG 3350). Add 100 mM DTT and filter sterilize. Store in 1 mL aliquots at -20 °C.