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From 2013.igem.org

Revision as of 10:59, 11 July 2013 (view source) Ariadni (Talk | contribs) (→Results) ← Older edit		Revision as of 11:08, 11 July 2013 (view source) Ariadni (Talk | contribs) (→Results) Newer edit →
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	* 11.7 ng/ul (CYC)		* 11.7 ng/ul (CYC)
-	Pictures from gels	+	===Pictures from gels===
		+	Gel AMO
		+
		+	[[File:2013-07-08_AMO colony PCR.jpg | 600px]]
	Gel HAO-CYC		Gel HAO-CYC

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Revision as of 11:08, 11 July 2013

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.4 Results 1.4.1 Pictures from gels

208

Main purpose

Run gel with 1 AMO, 10 AMO, 20 AMO genes from "Nitrosomonas Europaea". Purification and extraction of the gel for 10 AMO. Run 2nd gel with all the sample of 10 AMO. Run gel with HAO and CYC genes "Nitrosomonas Europaea".

Who was in the lab

Kristian, Ariadni, Gosia

Procedure

Run 1st gel (AMO):

• 1ul of dye
• 5ul of sample
• 5ul Kb ladder

Purification of first gel for 10 AMO according to the protocol QIA gel extraction protocol. (given in the Kit)

Run 2nd gel (AMO):

• 9ul of dye
• around 20 ul of each sample
• 12 ul of ladder

Purification of second gel according to the same protocol.

Measure the concentration of the purified AMO using Nanodrop (pouring together both samples).

Run 3rd gel (HAO,CYC):

• 1ul of dye
• 5ul of sample
• 5ul Kb ladder

Purification of DNA of both HAO and CYC.

Results

Nanodrop measurement:

• 30.4 ng/ul (AMO)
• 14.4 ng/ul (HAO)
• 11.7 ng/ul (CYC)

Pictures from gels

Gel AMO

Gel HAO-CYC

Wells:

• 1: 1 Kb Plus DNA Ladder
• 2: HAO
• 3: HAO
• 4: HAO
• 5: HAO
• 6: HAO
• 7: HAO

Around 3000 basepairs

• 8: CYC
• 9: CYC
• 10: CYC
• 11: CYC
• 12: CYC
• 13: CYC

Around 1650 basepairs

• 14: 1 Kb Plus DNA Ladder