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Team:NTNU-Trondheim/Notebook/October
From 2013.igem.org
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Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below | ||
| - | <div class="col4" style="background-color:white;><a href=" "> <img src=" " width="303"> | + | <div class="col4" style="background-color:white;><a href="https://static.igem.org/mediawiki/2013/8/8e/Bakteri_SDS_gel_2.jpg"> <img src="https://static.igem.org/mediawiki/2013/8/8e/Bakteri_SDS_gel_2.jpg" width="303"> |
| - | <p style="text-align:center; color:black; "> Figure 1.</p> </div> | + | <p style="text-align:center; color:black; "> <b>Figure 1:</b> SDS-PAGE of bacterial samples. From the left: wild type ER2566, tat_GFP_RFP and tat_ProteinG</p> </div> |
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| - | There is evidently produced Protein G in the S3-2B sample as there is an additional band at the expected size of about | + | There is evidently produced Protein G in the S3-2B sample as there is an additional band at the expected size of about 60 kDa. There is also an additional band at the ER1 sample of about 28-30 kDa. This half the expected size of about 60 kDa. This indicates that only RFP is produced in the bacterias.<br></p> |
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Revision as of 10:36, 4 October 2013
Wednesday 02.10.2013
SDS-PAGE of bacterial samples with the tat_GFP_RFP (ER1) and tat_ProteinG construct
In order to determine if Protein G is produced in the S3-2B sample and wheater a GFP-RFP dimer is produced in the ER1 sample, a SDS-PAGE was performed. Liquid cell culture with ER1 (tat_GFP_RFP construct), S3-2B (tat_ProteinG construct) and wildtype ER2566 ''E.coli'' cells was centrifuged and the pallet was mixed with SDS loading buffer. This mix was then heated at 95 °C for 15 minutes. The SDS-PAGE results can be viewed in the figure below
Figure 1: SDS-PAGE of bacterial samples. From the left: wild type ER2566, tat_GFP_RFP and tat_ProteinG
