"
Team:Tuebingen/Notebook/Protocols/pgem-ligation
From 2013.igem.org
(Difference between revisions)
| Line 51: | Line 51: | ||
<p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).</p> | <p>Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).</p> | ||
| - | + | ||
<a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | <a href="https://2013.igem.org/Team:Tuebingen/Notebook/Protocols" style="font-size: 18px">Back to Protocols</a> | ||
</div> | </div> | ||
Latest revision as of 12:09, 4 October 2013
pGEM Ligation
Reagents
| 5 µL | 2x Rapid Ligation Buffer |
| 0.5 µL (25 ng) | pGEM vector |
| 3.5 µL | PCR |
| 10 mL | KCl (c = 250 mM) |
Procedure
Dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
Back to Protocols
