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Team:DTU-Denmark/Notebook/8 September 2013
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Latest revision as of 12:12, 4 October 2013
8 September
Contents |
lab 208
Who was in the lab
Henrike, Kristian, Julia
Procedure
Colony PCR to verify Nir in pZA21::ara-tight
Colony PCR on the Nir transformants, testing for NirH (primers 45a, 45b, annealing 59C), NirM+S (primers 44, 11a annealing 64C) and NirN (primers 43, 11b annealing 68C).
program:
| temperature | time | cycles |
|---|---|---|
| 98C | 10:00 | - |
| 98C | 0:10 | 36 |
| 59C/64C/68C | 0:30 | 36 |
| 72C | 2:00 | 36 |
| 72C | 5:00 | - |
| 10C | hold | - |
Colony PCR to verify HAO insert.
Primers: 28a, 28b
temperature: 59C
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