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Team:SydneyUni Australia/Project
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== '''Overall project''' == | == '''Overall project''' == | ||
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| + | '''Background''' | ||
| + | *1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty. | ||
| + | *DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world. | ||
| + | *Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective. | ||
| + | *A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1). | ||
| + | '''Goals''' | ||
| + | *Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2. | ||
| + | *Construct and compare two of the proposed pathways of DCA biodegradation. | ||
| + | *Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts. | ||
| + | *(If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination. | ||
== Project Details== | == Project Details== | ||
Revision as of 12:03, 15 July 2013
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Contents |
Overall project
Background
- 1,2-dichloroethane (DCA) is part of a family of chlorinated hydrocarbons that are primarily derived from industrial solvents such as tetrachloroethene (PCE) and trichloroethene (TCE). Some of these organochlorines are toxic, carcinogenic and generally nasty.
- DCA is a soluble and mobile contaminant of the groundwater in Botany Bay, Sydney, but also elsewhere around the world.
- Conventional treatment involves significant costs, such as the pumping and heat-stripping of groundwater at Botany Bay. A biological alternative may be cheaper and more effective.
- A suite of techniques (including sampling of contaminated sites, growth in bioreactors with selective conditions and protein engineering) have led to an understanding that there are two primary pathways of DCA-degradation (Fig. 1).
Goals
- Construct a BioBrick-compatible vector inspired by the broad host range vector, pBBR1MCS2.
- Construct and compare two of the proposed pathways of DCA biodegradation.
- Characterise the components of the DCA-degradation pathway for admission into the Registry of Standard Parts.
- (If we get time!) Demonstrate the integration of our the pathway in the chromosome of Pseudomonas stutzeri via natural transformation and site-specific recombination.
