Work done by the team

30px Katarzyna Chyzynska

• Performed characterization and verification experiments
• Designed our poster
• Participated in high school outreach and the Biobrick workshop

Helen Cook

• Performed and analyzed data from the characterization and verification experiments
• Created the diagrams describing our project
• Participated in high school outreach, Biobrick workshop and the discussion on IP and synthetic biology.

30px Kristian Davidsen

• Lead user cloning work in the 208 lab; designed primers
• Constructed the pBAD_SPL
• Found sponsors and funding
• Starred in our bricks of knowledge video on USER cloning
• Participated in high school outreach and the Biobrick workshop

Ariadni Droumpali

• Performed characterization and verification experiments
• Participated in high school outreach, Biobrick workshop and the discussion on IP and synthetic biology.

30px Piotr Dworzynski

• Simulation of GFP in the periplasm for Hello World project

30px Malgorzata Futyma

• USER cloning and other work in 208 lab
• Drew pictures for our banner and front page slideshow
• Participated in high school outreach and the Biobrick workshop

30px Jakob Berg Jespersen

• Constructed protein models
• Designed our t-shirts and business cards
• Found sponsors and funding
• Participated in high school outreach and the Biobrick workshop

30px Vanessa Jurtz

• Modeled kinetics of our reactions
• Modeled a continuous flow reactor
• Participated in high school outreach and the Biobrick workshop

Anastasia Mourka

• Wiki design and implementation
• Participated in the discussion on IP and synthetic biology.

Natalia Papargyri

• Responsible for our safety form and section
• Participated in high school outreach and the Biobrick workshop

Julia Villarroel

• USER cloning in 208 lab
• Participated in high school outreach and the Biobrick workshop

30px Henrike Zschach

• USER cloning in the 208 lab
• Participated in high school outreach, Biobrick workshop and the discussion on IP and synthetic biology.

Assistance provided by our supervisors

Chris Workman (PI)

• Provided perl and R scripts to analyze raw biolector data
• Provided lab space for us to work in (building 208)

Barth Smets

• Provided the idea for our project
• Provided lab space for us to work in (building 115)

Assistance provided by our advisors

30px Thomas Trolle

• Provided early advice on the design of the pBAD SPL
• Helped during the BioBrick workshop

30px Anne Mathilde Lund

• Generously provided us with X7 polymerase and assistance with debugging PCR reactions
• Arranged all experiments under the BioBrick workshop
• Great help with primer design for USER cloning as with introduction on how to use the gradient PCR-machine (link to notebook)
• Helped in "hard to amplify" PCR situations

30px Andreas Porse

• Reviewed our initial plan and provided insight into E. coli specific signal peptides
• Assisted in primer design for USER cloning i the "Hello World project"
• General questions about sequencing, PCR, lab techniques etc.

30px Julie Rank

• Viewed early versions of our presentation and provided excellent feedback on how we could improve
• Helped during the BioBrick workshop

Ali Altıntaş

• Provided assistance in the 208 lab with general lab technique
• Help with debugging gel pictures and methods
• Kindly gave us filter sterilization units

Sébastien Muller

• Provided assistance in the 208 lab with general lab technique
• Helped debugging PCR-reactions
• Kindly gave us loading dye and DNA ladder

Special Thanks to

[http://www.env.dtu.dk/english/Research/RRE/RRE-Staff/Person?id=66486&cpid=86362&tab=2&qt=dtupublicationquery| Gizem Mutlu], PhD student DTU Environmental Engineering for her help with initial calibration of the N₂O and NO sensors, and for preparing the NO standard solution.

Tina Johansen, Laboratory Technician DTU Systems biology for her technical help with the bioreactor set up and to [http://www.dtu.dk/Service/Telefonbog/Person?id=7254&cpid=710&tab=2&qt=dtupublicationquery| Jette Thykær], Associate Professor DTU Systems Biology for her assistance scheduling time on the bioreactor.

Natalia Skawińska, who participated in the high school outreach, and provided advice on cloning in E. coli.

Morten Nørholm, who developed the X7 polymerase [http://www.biomedcentral.com/1472-6750/10/21].

[http://www.cbs.dtu.dk/ Center for Biological Sequence Analysis] for letting us use one of their offices and their coffee machine!

DTU iGEM 2011 Team for their Wiki Guide, which helped in the creation of this site.

DTU iGEM 2009 Team for their software, [http://www.cbs.dtu.dk/services/PHUSER/ PHUSER 2.0], that we have used and beta tested.

University of Copenhagen iGEM 2013 Team and University of Southern Denmark iGEM 2013 Team for joining us for the Biobrick Workshop and indian food.

University of Southern Denmark iGEM 2013 Team for arranging the DK-Meet-up.

Sponsors

	Otto Mønsted Fonden
	Otto Bruuns Fond
	Videncenteret for Landbrug
	Brenntag
	Krüger
	DTU Systems Biology
	DTU Environment
	Technical University of Denmark
	CLC Bio
	New England Biolabs GmbH
	Kailow