"
Team:DTU-Denmark/Notebook/12 July 2013
From 2013.igem.org
(Difference between revisions)
(→Procedure) |
(→Amplification of cytochromes, AMO, HAO and Nir genes) |
||
| Line 14: | Line 14: | ||
Samples are named: | Samples are named: | ||
| + | *1, 2, 3 -> cytochromes | ||
| + | *4, 5, 6 -> AMO | ||
| + | *7, 8, 9 -> HAO | ||
| + | *10, 11, 12 -> Nir | ||
Revision as of 14:20, 12 July 2013
Contents |
208
Main purpose
- miniprep of biobrick transformants from 10-07-2013
- PCR with USER primers to amplify cytochromes, AMO, HAO and Nir genes
- gel analysis of PCR products from Nir operon combined with pZA21 and activity test of inducible AraBAD promoter
Who was in the lab
Henrike, Julia, Kristian, Jakob, Gosia
Procedure
Amplification of cytochromes, AMO, HAO and Nir genes
Each PCR reaction was performed in triplicate.
Samples are named:
- 1, 2, 3 -> cytochromes
- 4, 5, 6 -> AMO
- 7, 8, 9 -> HAO
- 10, 11, 12 -> Nir
