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Team:DTU-Denmark/Notebook/12 July 2013
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*PCR with USER primers to amplify cytochromes, AMO, HAO and Nir genes | *PCR with USER primers to amplify cytochromes, AMO, HAO and Nir genes | ||
*gel analysis of PCR products from [[Team:DTU-Denmark/Notebook/11_July_2013#Combine_Nir_operon_with_pZA21|Nir operon]], [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| AraBAD promoter]] and [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| RFP]] | *gel analysis of PCR products from [[Team:DTU-Denmark/Notebook/11_July_2013#Combine_Nir_operon_with_pZA21|Nir operon]], [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| AraBAD promoter]] and [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| RFP]] | ||
| - | * USER reaction of [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| RFP]] and pZA21 (with native promoter) and transformation of | + | * USER reaction of [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| RFP]] and pZA21 (with native promoter) and transformation of ''E. coli'' cells |
==Who was in the lab== | ==Who was in the lab== | ||
Revision as of 15:24, 12 July 2013
Contents |
208
Main purpose
- miniprep of biobrick transformants from 10-07-2013
- PCR with USER primers to amplify cytochromes, AMO, HAO and Nir genes
- gel analysis of PCR products from Nir operon, AraBAD promoter and RFP
- USER reaction of RFP and pZA21 (with native promoter) and transformation of E. coli cells
Who was in the lab
Henrike, Julia, Kristian, Jakob, Gosia
Procedure
Amplification of cytochromes, AMO, HAO and Nir genes
Each PCR reaction was performed in triplicate.
Samples are named:
- 1, 2, 3 -> cytochromes
- 4, 5, 6 -> AMO
- 7, 8, 9 -> HAO
- 10, 11, 12 -> Nir
