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Team:NTNU-Trondheim/Primers
From 2013.igem.org
(Difference between revisions)
| Line 174: | Line 174: | ||
<tr> | <tr> | ||
<td><b>F_l_YFP</tr></b></td> | <td><b>F_l_YFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_YFP</tr></b></td> | <td><b>R_pl.b_YFP</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3'</td> |
</tr> | </tr> | ||
| Line 185: | Line 185: | ||
<tr> | <tr> | ||
<td><b>F_pl.b_PrG</tr></b></td> | <td><b>F_pl.b_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_tat_PrG</tr></b></td> | <td><b>F_tat_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_pl.b_pelB_PrB</tr></b></td> | <td><b>F_pl.b_pelB_PrB</tr></b></td> | ||
| - | <td></td> | + | <td>5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCgttgactcaccaatcgaagatacccc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_PrG</tr></b></td> | <td><b>R_pl.b_PrG</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b_PrGstop</tr></b></td> | <td><b>R_pl.b_PrGstop</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3'</td> |
</tr> | </tr> | ||
| Line 208: | Line 208: | ||
<tr> | <tr> | ||
<td><b>F_plasmid </tr></b></td> | <td><b>F_plasmid </tr></b></td> | ||
| - | <td></td> | + | <td>5'-ccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_RFP_pl.b</tr></b></td> | <td><b>F_RFP_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>F_YFP_pl.b</tr></b></td> | <td><b>F_YFP_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_pl.b</tr></b></td> | <td><b>R_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'- ctagtatttctcctctttctctagtagtgc-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_tat_pl.b</tr></b></td> | <td><b>R_tat_pl.b</tr></b></td> | ||
| - | <td></td> | + | <td>5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3'</td> |
</tr> | </tr> | ||
| Line 231: | Line 231: | ||
<tr> | <tr> | ||
<td><b>F_sequence</tr></b></td> | <td><b>F_sequence</tr></b></td> | ||
| - | <td></td> | + | <td>5'-tgccacctgacgtctaagaa-3'</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><b>R_sequence</tr></b></td> | <td><b>R_sequence</tr></b></td> | ||
| - | <td></td> | + | <td>5'-attaccgcctttgagtgagc-3'</td> |
</tr> | </tr> | ||
Revision as of 19:31, 4 October 2013
Primers
| tat-signal sequence | |
|---|---|
| Primer | Sequence |
| F_pl.b_tat | 5'- AGAGAAAGAGGAGAAATACTAGatggccaataacgatctctttcaggcatcacg-3' |
| F_prefix_tat | 5'- GAATTCGCGGCCGCTTCTAGatggccaataacgatctctttcaggcatcacg-3' |
| R_tat | 5'-cgcttgcgccgcagtcgcacgtcg-3' |
| R_GFP_tat | 5'-CTTCTCCTTTACGCATcgcttgcgccgcagtcgcacgtcg -3' |
| R_CFP_tat | 5'-GCCCTTGCTCACCATcgcttgcgccgcagtcgcacgtcg-3' |
| SYFP | |
| F_I_syfp | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggttagcaagggcgaaga-3' |
| R_syfp | 5'-GCCTTTCGTTTTATTTGATGCCTGGttattatttatacagctcatccatacccaggg-3' |
| BFP | |
| F_tat_BFP | 5'- CGACGTGCGACTGCGGCGCAAGCGatgagcgaactgatcaaagag-3' |
| R_I_bfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCattcagtttatgacccagcttgc-3' |
| GFP | |
| F_tat_GFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatgcgtaaaggagaagaact-3' |
| R_I_gfp | 5'-ACTACCACCGGATCCACCTGATCCACCGGATCCACCtttgtatagttcatccatgcc-3' |
| RFP | |
| F_I_rfp | 5'- GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggcttcctccgaagacg-3' |
| R_pl.b_rfp | 5'- GCCTTTCGTTTTATTTGATGCCTGGgcgatctacactagcactatcagcg-3' |
| GFPmut3 | |
| F_tat_GFPmut3 | 5'-CGACGTGCGACTGCGGCGCAAGCGagaaaaggagaagaacttttcactgg-3' |
| R_pl.b_GFPmut3 | 5-'GCCTTTCGTTTTATTTGATGCCTGGttatttgtatagttcatccatgcc-3' |
| CFP | |
| F_tat_CFP | 5'-CGACGTGCGACTGCGGCGCAAGCGatggtgagcaagggcgaggagctgttc-3' |
| R_l_CFP | 5'- ACTACCACCGGATCCACCTGATCCACCGGATCCACCcttgtacagctcgtccatgccgagag-3' |
| YFP | |
| F_l_YFP | 5'-GGTGGATCCGGTGGATCAGGTGGATCCGGTGGTAGTatggtgagcaagggcgaggagctgttc-3' |
| R_pl.b_YFP | 5'- GCCTTTCGTTTTATTTGATGCCTGGttattacttgtacagctcgtccatgccg-3' |
| Protein G | |
| F_pl.b_PrG | 5'-AGAGAAAGAGGAGAAATACTAGatggaaaaagaaaaaaaggtaaaatac-3' |
| F_tat_PrG | 5'-CGACGTGCGACTGCGGCGCAAGCGgttgactcaccaatcgaagatacccc-3' |
| F_pl.b_pelB_PrB | 5'-AGAGAAAGAGGAGAAATACTAGATGAAATACCTGCTGCCGACCGCTGCTGCTGGTCTGCTGCTCCTCGCTGCCCAGCCGGCGATGGCCgttgactcaccaatcgaagatacccc-3' |
| R_pl.b_PrG | 5'- GCCTTTCGTTTTATTTGATGCCTGGgatcggcatattgaaaaggc-3' |
| R_pl.b_PrGstop | 5'-GCCTTTCGTTTTATTTGATGCCTGGttagtcttctttacgttttgaagcgac-3' |
| Plasmid backbone | |
| F_plasmid | 5'-ccaggcatcaaataaaacgaaaggc-3' |
| F_RFP_pl.b | 5'-GTGCTAGTGTAGATCGCccaggcatcaaataaaacgaaaggc-3' |
| F_YFP_pl.b | 5'-GAGCTGTACAAGTAATAAccaggcatcaaataaaacgaaaggc-3' |
| R_pl.b | 5'- ctagtatttctcctctttctctagtagtgc-3' |
| R_tat_pl.b | 5'- GAGATCGTTATTGTTCATctagtatttctcctctttctctagtagtgc-3' |
| Sequencing Primers | |
| F_sequence | 5'-tgccacctgacgtctaagaa-3' |
| R_sequence | 5'-attaccgcctttgagtgagc-3' |
