Team:KU Leuven/Parts

From 2013.igem.org

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   <p align = "justify">Here you will find the BioBricks we made this summer in the wetlab. Our three favorite parts are marked with a heart:<br/>
   <p align = "justify">Here you will find the BioBricks we made this summer in the wetlab. Our three favorite parts are marked with a heart:<br/>
<ol>
<ol>
-
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060002">BBa_K1060002</a> was a milestone in our project work. We succeeded to clone the beta farnesene synthase of <i>Artemisia annua</i> in <i>E. coli</i>. To apply to the iGEM standards, we removed an <i>EcoRI</i> site in the gene (AY835398.1).</li><br />
+
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060000">BBa_K1060000</a> contains the aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also BBa_K1060003). As basic chorismate levels inside <i>E. coli</i> are limited. We wanted to upregulate the biosynthesis of chorismate. The basic aroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.</li><br/>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060001">BBa_K1060001</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060002">BBa_K1060002</a> was a milestone in our project work. We succeeded to clone the beta farnesene synthase of <i>Artemisia annua</i> in <i>E. coli</i>. To apply to the iGEM standards, we removed an <i>EcoRI</i> site in the gene (AY835398.1).</li><br/>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060003">BBa_K1060003</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060004">BBa_K1060004</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060005">BBa_K1060005</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060006">BBa_K1060006</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060007">BBa_K1060007</a>
 +
 
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060008">BBa_K1060008</a>
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060009">BBa_K1060009</a> is a construct that constitutively expresses beta farnesene synthase. This synthase will convert farnesyl pyrophosphate into the aphid alarm hormone, (E)-β-farnesene.</li><br />
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060009">BBa_K1060009</a> is a construct that constitutively expresses beta farnesene synthase. This synthase will convert farnesyl pyrophosphate into the aphid alarm hormone, (E)-β-farnesene.</li><br />
 +
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060010">BBa_K1060010</a>
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060011">BBa_K1060011</a> is similar to BBa_K1060009. However, in this biobrick we added a <i>lac</i> operator in front of the beta farnesene synthase. This makes it possible to switch of (E)- β-farnesene production by using biosensors expressing LacI.</li><br />
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060011">BBa_K1060011</a> is similar to BBa_K1060009. However, in this biobrick we added a <i>lac</i> operator in front of the beta farnesene synthase. This makes it possible to switch of (E)- β-farnesene production by using biosensors expressing LacI.</li><br />
 +
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060012">BBa_K1060012</a>
 +
 +
<li> <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1060013">BBa_K1060013</a>
</ol>
</ol>
   </html><groupparts>iGEM013 KU_Leuven</groupparts><html>
   </html><groupparts>iGEM013 KU_Leuven</groupparts><html>

Revision as of 23:29, 4 October 2013

iGem

Secret garden

Congratulations! You've found our secret garden! Follow the instructions below and win a great prize at the World jamboree!


  • A video shows that two of our team members are having great fun at our favourite company. Do you know the name of the second member that appears in the video?
  • For one of our models we had to do very extensive computations. To prevent our own computers from overheating and to keep the temperature in our iGEM room at a normal level, we used a supercomputer. Which centre maintains this supercomputer? (Dutch abbreviation)
  • We organised a symposium with a debate, some seminars and 2 iGEM project presentations. An iGEM team came all the way from the Netherlands to present their project. What is the name of their city?

Now put all of these in this URL:https://2013.igem.org/Team:KU_Leuven/(firstname)(abbreviation)(city), (loose the brackets and put everything in lowercase) and follow the very last instruction to get your special jamboree prize!

tree ladybugcartoon

Here you will find the BioBricks we made this summer in the wetlab. Our three favorite parts are marked with a heart:

  1. BBa_K1060000 contains the aroG gene, coding for 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase and is a part of the Shikimate pathway. It converts phosphoenolpyruvate and erythrose-4P to chorismate. In our project chorismate was one of the precursors needed for the biosynthesis of methyl salicylate (see also BBa_K1060003). As basic chorismate levels inside E. coli are limited. We wanted to upregulate the biosynthesis of chorismate. The basic aroG gene can be repressed by phenylalanine. However, mutatations in the amino acid sequence (Pro150Leu and Leu175Asp) can prevent this repression by phenylalanine.

  2. BBa_K1060001
  3. BBa_K1060002 was a milestone in our project work. We succeeded to clone the beta farnesene synthase of Artemisia annua in E. coli. To apply to the iGEM standards, we removed an EcoRI site in the gene (AY835398.1).

  4. BBa_K1060003
  5. BBa_K1060004
  6. BBa_K1060005
  7. BBa_K1060006
  8. BBa_K1060007
  9. BBa_K1060008
  10. BBa_K1060009 is a construct that constitutively expresses beta farnesene synthase. This synthase will convert farnesyl pyrophosphate into the aphid alarm hormone, (E)-β-farnesene.

  11. BBa_K1060010
  12. BBa_K1060011 is similar to BBa_K1060009. However, in this biobrick we added a lac operator in front of the beta farnesene synthase. This makes it possible to switch of (E)- β-farnesene production by using biosensors expressing LacI.

  13. BBa_K1060012
  14. BBa_K1060013
<groupparts>iGEM013 KU_Leuven</groupparts>

Retrieved from "http://2013.igem.org/Team:KU_Leuven/Parts"