"
Team:Groningen/Lab/experiments/Biofilm
From 2013.igem.org
(Difference between revisions)
| Line 34: | Line 34: | ||
<p> | <p> | ||
| - | The suggested implant material was placed in a petri dish with | + | The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate <B.subtilis</i> strain. After incubation the plate is left in a 27°C environment for 5 days. After the incubation time, the total biofilm growth on the material of interest is measured. |
<br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2). | <br>Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2). | ||
Latest revision as of 23:43, 4 October 2013
Biofilm Growth on different materials
To find out if the Bacillus subtilis strain used in this project can form a biofilm on material often used as implants, an experiment was set up.
The suggested implant material was placed in a petri dish with Sgg medium. The material is partially submerged in the medium and inoculated with the appropriate
Two different implant materials are tested: titanium and plastic. After five days of growth on titanium, there is 3 mm biofilm, starting at the medium growing up on titanium (Figure 1). No biofilm formation was possible on plastic (Figure 2).
|
| Figure 1: Titanium plate with 3 mm of biofilm production. |
|
| Figure 2: No biofilm production was seen on plastic. |
iGEM 2013 Groningen
|
|
|
|
 |
|
