Team:Heidelberg/Templates/Materials list

From 2013.igem.org

Revision as of 23:34, 4 October 2013

Materials used during the project

Partly adopted from iGEM Team Heidelberg 2009 and 2010. Please recheck.

Table 1: Kits

Table 2: Marker

Table 3: Enzymes

Table 4: Bacteria

Table 5: Media and Antibiotics

preparation of M9 minimal media according to Serva company

Tables 6: Primers and Oligos

Table 6A: Miscellaneous Primer

Table 6B: Delftibactin

Table 6C: Module Shuffling

Table 6D: Linker Variation

Table 6E: Domain Shuffling and PPTases

Table 7: Instruments

Table 8: Mass spectrometry at neonate screening

Solution 1 (for MS sample preparation): Neo Gen stable isotope standard kit A und B (NSK-AB, EurIsotop)
1. One vial of amino acid standard (NSK-A) is taken up in 1 ml methanol/water (1:1 v/v) and solubilized for ca. 15 minutes in an ultra-sonification bath. Caution: make sure that the lid is closed well and not wetted by water of the ultra-sonification bath.
2. One vial of acylcarnitine standard (NSK-B) is taken up in 1ml of methanol and solubilized for ca. 15 minutes in the ultra-sonification bath.Caution: make sure that the lid is closed well and not wetted by water of the ultra-sonification bath.
Contents of both vials are transfered quantitatively with methanol in a 200 ml graduate flask. The graduate flask is filled up to the calibration mark with methanol. Standard solutions get a consecutive number. When two 200 ml graduate flasks are prepared simultaneously, contents of both flasks can be combined, if levels of both standards (old and new) coincide. Afterwards the whole solution will be labeled with one common number.
Stability of solution 1: 7 days

Solution 2 (for MS sample preparation): butanolic hydrochloric acid (3 N), water-free:
1. 900 ml butanol (p.A., Merck 1.01990.1000) are cooled in an ice bath at 0°C (magnetic stirrer). The vessel has to be closed to avoid ice condensation of water.
2. 100 ml acetylchloride (p.A., Merck 1.00031.0250) are added dropwiese from a dropping funnel over a a period of 5 minutes while stirring constantly (protection goggles and gloves!).
Caution: make sure that no water or ice gets into the solution.
3. The solution is stirred for additional 10 minutes with closed lid on ice. Stability of solution 2: 1 year

Solution 1 (for MS sample preparation):
Acetonitrile p.A./water (1:1 v/v) (83639.320DE ACETONITRIL HIPERSOLV SUPER GR REAG.PE)
Stability of solution 3: 1 year

Table 9: Lab materials

Table 10: Plasmides

Table 11: Buffers

Table 12: other chemicals