Team:Groningen/protocols/Digestion

From 2013.igem.org

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<br>When the reaction mix is ready, the content is mixed and incubated at 37&deg;C for 1h.
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<br>When the reaction mix is ready, the content is mixed and incubated at 37&deg;C for 1h [1].
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<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf
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<br>When the reaction mix is ready, the content is mixed and incubated at 37&deg;C for 2h.
<br>When the reaction mix is ready, the content is mixed and incubated at 37&deg;C for 2h.
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<br> [1]Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf
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Revision as of 23:32, 4 October 2013

Restriction digestion

Fast digest

Materials:
  • MQ water
  • 1.5 ml tubes
  • Fast digestion buffer
  • Restriction enzymes
  • DNA
Reaction:
Compound 20 µl Final concentration
MQ water up to 20 µl
10x fast digestion buffer 2 µl 1x
restriction enzyme(s) 1 µl 1U
DNA 4 µl up to 1 µg

When the reaction mix is ready, the content is mixed and incubated at 37°C for 1h [1].


Normal digest

Materials:
  • MQ water
  • 1.5 ml tubes
  • Enzyme digestion buffer
  • Restriction enzymes
  • DNA
Reaction:
Compound 20 µl Final concentration
MQ water up to 20 µl
10x restriction digestion buffer 2 µl 1x
restriction enzyme(s) 0.5 µl 1U
DNA 2 µl 0.2-1.5 µg

When the reaction mix is ready, the content is mixed and incubated at 37°C for 2h.

[1]Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/fast-digestion-dna.pdf