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Team:Wageningen UR/Summary
From 2013.igem.org
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<p>Concluding our iGEM project in 2013, we introduced <i>Aspergillus niger</i> as a potential host into iGEM. It is an industrially relevant organism, deserving a place in iGEM as a standard synthetic biological chassis. With the host engineering, we stepped further to the next level of synthetic biology.</p> | <p>Concluding our iGEM project in 2013, we introduced <i>Aspergillus niger</i> as a potential host into iGEM. It is an industrially relevant organism, deserving a place in iGEM as a standard synthetic biological chassis. With the host engineering, we stepped further to the next level of synthetic biology.</p> | ||
| - | <p>In this GRAS organism, we established a modular system of domain shuffling in order to express a variety of secondary metabolites. We focused on the production of a medically relevant compound, lovastatin, which has never been produced in <i>A. niger</i>. | + | <p>In this GRAS organism, we established a modular system of domain shuffling in order to express a variety of secondary metabolites. We focused on the production of a medically relevant compound, lovastatin, which has never been produced in <i>A. niger</i>.We synthesized a range of modules for the main multi-domain enzyme in the lovastatin pathway, developed a new frameshiftless assembly protocol to design new multidomain enzyme complexes, and bricked these parts for future teams.<p> |
| - | <p>Additionally, to target the production | + | <p>Additionally, to target the production in a specific compartment, a toolkit including a <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023001" target="_blank"> promoter</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023002" target="_blank"> terminator</a>, biosensors for <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023004" target="_blank"> pH</a>, <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023003 " target="_blank"> ATP</a>, and <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023005" target="_blank"> chromogenic biomarkers</a>, as well as a marker for the <a href=" http://parts.igem.org/wiki/index.php?title=Part:BBa_K1023006" target="_blank"> cytoskeleton</a> was created. It paved the way for future teams to further exploit this beautiful bug.</p> |
| - | <p>Finally, we modified the host by directed evolution. Filamentous fungi are pretty organisms, but multicellularity is not always wanted in fermentations. We set out to | + | <p>Finally, we modified the host by directed evolution. Filamentous fungi are pretty organisms, but multicellularity is not always wanted in fermentations. We set out to investigate it by evolving mutants with a reduced mycelial cohesiveness. Microscopic analyses have shown that <i>Aspergillus</i> is conditionally dimorphic, and both viable and metabolically active in a single cell morphotype. We made important steps in unraveling the causes of multicellularity. </p> |
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| + | <p>All in all, our project has been ambitious, inspiring, educational, social, and totally worthwhile. We made mistakes, underestimated some experimental time frames, totally failed in some experimental set ups, but nothing beats the excitement of positive results.<p> | ||
Revision as of 00:53, 5 October 2013
- Safety introduction
- General safety
- Fungi-related safety
- Biosafety Regulation
- Safety Improvement Suggestions
- Safety of the Application
Summary
We made mistakes,
though this whole endeavor was incredibly worthwhile.
We learned a lot,
and nothing beats the excitement of positive results.
Concluding our iGEM project in 2013, we introduced Aspergillus niger as a potential host into iGEM. It is an industrially relevant organism, deserving a place in iGEM as a standard synthetic biological chassis. With the host engineering, we stepped further to the next level of synthetic biology.
In this GRAS organism, we established a modular system of domain shuffling in order to express a variety of secondary metabolites. We focused on the production of a medically relevant compound, lovastatin, which has never been produced in A. niger.We synthesized a range of modules for the main multi-domain enzyme in the lovastatin pathway, developed a new frameshiftless assembly protocol to design new multidomain enzyme complexes, and bricked these parts for future teams.
Additionally, to target the production in a specific compartment, a toolkit including a promoter, terminator, biosensors for pH, ATP, and chromogenic biomarkers, as well as a marker for the cytoskeleton was created. It paved the way for future teams to further exploit this beautiful bug.
Finally, we modified the host by directed evolution. Filamentous fungi are pretty organisms, but multicellularity is not always wanted in fermentations. We set out to investigate it by evolving mutants with a reduced mycelial cohesiveness. Microscopic analyses have shown that Aspergillus is conditionally dimorphic, and both viable and metabolically active in a single cell morphotype. We made important steps in unraveling the causes of multicellularity.
All in all, our project has been ambitious, inspiring, educational, social, and totally worthwhile. We made mistakes, underestimated some experimental time frames, totally failed in some experimental set ups, but nothing beats the excitement of positive results.
