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Team:Paris Saclay/Notebook/June/28
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=='''Lab work'''== | =='''Lab work'''== | ||
| - | 1 | + | ==='''Objective : obtaining BBa_K1155000'''=== |
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| + | ===='''1 - PCR for the sequence of promoter Pndh* and origin of replication''' ==== | ||
- Make an agarose gel with BET | - Make an agarose gel with BET | ||
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- Gel electrophoresis with PCR product | - Gel electrophoresis with PCR product | ||
| - | + | ==== '''2 - Test efficiency of the super competent cell''' ==== | |
| - | + | ||
| - | 2 | + | |
- transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes) | - transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes) | ||
Revision as of 01:02, 5 October 2013
Contents |
Notebook : June 28
Lab work
Objective : obtaining BBa_K1155000
1 - PCR for the sequence of promoter Pndh* and origin of replication
- Make an agarose gel with BET
- Gel electrophoresis with PCR product
2 - Test efficiency of the super competent cell
- transformation super competent cells with plasmid PUC18. (Spread bacteria on the boxes)
Protocol : Bacterial tranformation
- Plate bacteria on LB plate with ampicillin
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