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Template:Team:Bonn:NetworkData
From 2013.igem.org
(Difference between revisions)
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content.childs=[]; | content.childs=[]; | ||
content.titleShort = "Generated plasmids"; | content.titleShort = "Generated plasmids"; | ||
| - | content.titleLong = " | + | content.titleLong = "A list of generated plasmids |
| - | content.summary= ""; | + | content.summary= "A list of each and every plasmid we designed combined with short summaries of their function"; |
| - | content.text=""; | + | content.text="<a href='https://static.igem.org/mediawiki/2013/e/ef/Bonn_AraC-pBAD%28D%29-RBS32-mazF-TT_BBa_K1075040.jpg'><img src='https://static.igem.org/mediawiki/2013/e/ef/Bonn_AraC-pBAD%28D%29-RBS32-mazF-TT_BBa_K1075040.jpg' width='840' height='200'></a>MazF is part of the biological toxin-antitoxin system MazEF. This system is derived from the bacterium Escherichia coli, where it serves as a stress inducable killswitch. MazF, a RNA-degrading enzyme, ist he toxin and is normally inhibited by the presence of MazE. Absence of MazE leads to cell death.<sup><a href=http://parts.igem.org/Part:BBa_K1075040>1</a></sup><a href='https://static.igem.org/mediawiki/2013/3/34/Bonn_pLac1-RBS32-mazF-TT_BBa_K1075039.jpg'><img src='https://static.igem.org/mediawiki/2013/3/34/Bonn_pLac1-RBS32-mazF-TT_BBa_K1075039.jpg' width='840' height='200'></a>We used this part to gain an IPTG and Lactose inducible construct of MazF. This part was used to test the toxicity of MazF as part of our proof of concept. <sup><a href=http://parts.igem.org/Part:BBa_K1075039>2</a></sup><a href='https://static.igem.org/mediawiki/2013/8/87/Bonn_pLac2-RBS32-mazE-%28ec%29ssrA%28DAS%2B4%29-TT_BBa_K1075043.jpg'><img src='https://static.igem.org/mediawiki/2013/8/87/Bonn_pLac2-RBS32-mazE-%28ec%29ssrA%28DAS%2B4%29-TT_BBa_K1075043.jpg' width='840' height='200'></a>This part can be used to gain a Lactose and IPTG inducable construct of mazE-(ec)ssrA(DAS+4). We used this construct as part of our proof of concept. <sup><a href=http://parts.igem.org/Part:BBa_K1075043>2</a></sup>"; |
content.type="Project"; | content.type="Project"; | ||
break; | break; | ||
Revision as of 02:54, 5 October 2013
