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Team:MSOE Milwaukee/Primers
From 2013.igem.org
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<TABLE> | <TABLE> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #006331 width = 200 valign = bottom height = 50><font color = "white" size = "+2" >DNA SEQUENCE/ENZYME</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #006331 width = 760 valign = bottom height = 50><font color = "white" >PRIMER TO ADD THE PREFIX (FOR) 5' to 3' <br> PRIMER TO ADD THE SUFFIX (REV) 5' to 3'</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">1,8-cineole synthase</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">HMG-CoA Synthase reverse</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align= left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Kinase</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white">ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Phosphomevalonate Kinase</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Mevalonate Diphosphate Kinase</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylanase C</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC<br>GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">Xylosidase</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG<br>GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG</TD> |
</TR> | </TR> | ||
<TR align = center> | <TR align = center> | ||
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50><font color = "white" size = "+2">osmY with GS liner (two reverse)</TD> |
| - | <TD bgcolor = # | + | <TD bgcolor = #009047 width = 320 valign = center height = 50 align = left><font color = "white" >ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG<br>GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT<br>GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC</TD> |
</TR> | </TR> | ||
</TABLE> | </TABLE> | ||
</HTML> | </HTML> | ||
Revision as of 14:13, 22 August 2013
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| DNA SEQUENCE/ENZYME | PRIMER TO ADD THE PREFIX (FOR) 5' to 3' PRIMER TO ADD THE SUFFIX (REV) 5' to 3' |
| 1,8-cineole synthase | ATCGGAATTCGCGGCCGCTTCTAGATGGCTACCCTGCGTATC GCTAATGATCATCGCCGGCGACGTCCTTATTAACCCAGACGGTTAACG |
| HMG-CoA Synthase reverse | ATCGGAATTCGCGGCCGCTTCTAGCATGACCATCGGTATCGAC GCTAATGATCATCGCCGGCGACGTCCTTATTAGTTACGGTAAGAACGAACG |
| Mevalonate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGACCAAAAAAGTTGGTGTTGG GCTAATGATCATCGCCGGCGACGTCCTTATTACAGAGATTCGATCCAGG |
| Phosphomevalonate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGATCGCTGTTAAAACCTGC GCTAATGATCATCGCCGGCGACGTCCTTATTAAGATTTGTCGTCGTGACC |
| Mevalonate Diphosphate Kinase | ATCGGAATTCGCGGCCGCTTCTAGCATGGACCGTGAACCGG GCTAATGATCATCGCCGGCGACGTCCTTATTAGCAGCAGTCGTCC |
| Xylanase C | ATCGGAATTCGCGGCCGCTTCTAGCATGTTCTGCTCTAACGCTCAGC GCTAATGATCATCGCCGGCGACGTCCTTATTAGGTCAGACCCATTTTTTCC |
| Xylosidase | ATCGGAATTCGCGGCCGCTTCTAGCATGAAATCTAAACTGCTGTTCCCG GCTAATGATCATCGCCGGCGACGTCTTATTACGGTTTTTTGGTCAGG |
| osmY with GS liner (two reverse) | ATCGGAATTCGCGGCCCGCTTCTAGATGACTATGACAAGACTGAAG GGATCCCCCGCTACCACTGCCCGAACCCTTAGTTTTCAGATCATT GCTAATGATCATCGCCGGCGACGTCGGATCCCCCGCTACCACTGCCC |
