Team:Heidelberg/Templates/DelH week25
From 2013.igem.org
(Difference between revisions)
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==ccdB== | ==ccdB== | ||
Annealing temps: </br> | Annealing temps: </br> | ||
- | 12 cycles 74, 68, 64 and 58°C < | + | 12 cycles 74, 68, 64 and 58°C <br/> |
18 cycles 72, 66, 62 and 56°C | 18 cycles 72, 66, 62 and 56°C |
Revision as of 15:26, 18 October 2013
(18.10)
Contents |
HM17-FS_78
Annealing temps: 62°C, 60°C, 57°C, 55°C
Elongation time: 2:10
--> Did not work
Screening remaining Gibson electroporated
60 colonies were picked and screened with primers FS_73 and FS_95 (annealing 66 touchdown, 62)
Gibson assembly
Reagent | Volume added (µl) |
---|---|
BB (FS..) | 0.61 |
DN11-FS66 | 1.00 |
FS_67-FS_68 | 1.35 |
FS_69-FS_70 | 6.34 |
FS_71-HM08 | 0.70 |
Gibson Master Mix 2x | 10 |
ccdB
Annealing temps: </br>
12 cycles 74, 68, 64 and 58°C
18 cycles 72, 66, 62 and 56°C