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Exeter/2 July 2013
From 2013.igem.org
| Line 3: | Line 3: | ||
For one litre of broth we need... | For one litre of broth we need... | ||
| - | - | + | - 10 g tryptone |
| - | - | + | - 5 g yeast extract |
| - | - | + | - 5 g NaCl |
These were weighed out using a standard tabletop scale, accurate to 2 decimal places. | These were weighed out using a standard tabletop scale, accurate to 2 decimal places. | ||
| - | Add to ~ | + | Add to ~800 ml of distilled water and mix using a magnetic stirrer. When dissolved, add more distilled water to bring the overall amount to 1000 ml (1 litre) |
| - | Pour into | + | Pour into 200 ml Duran bottles, and add 3g of agar powder to each. This will not dissolve until the bottle is autoclaved for 20 minutes at 120oC. |
| - | We also made several | + | We also made several 50 ml batches of LB broth, stored in conical flasks. |
'''Learn how to make SOB growth media''' | '''Learn how to make SOB growth media''' | ||
| Line 21: | Line 21: | ||
For one litre of SOB we need... | For one litre of SOB we need... | ||
| - | - | + | - 5 g yeast extract |
| - | - | + | -20 g tryptone |
| - | - 0. | + | - 0.584 g NaCl |
| - | - 0. | + | - 0.186 g KCl |
| - | - 2. | + | - 2.4 g MgSO4 |
The tryptone, yeast extract and MgSO4 were weighed out using a standard tabletop scale, accurate to 2 decimal places. The NaCl and KCl were weighed out using a scale accurate to 4 decimal places. | The tryptone, yeast extract and MgSO4 were weighed out using a standard tabletop scale, accurate to 2 decimal places. The NaCl and KCl were weighed out using a scale accurate to 4 decimal places. | ||
| - | Dissolve into ~ | + | Dissolve into ~800 ml of distilled water using a magnetic stirrer, then add more distilled water until the overall volume is 1 litre. |
'''Make 20% weight by volume glucose solution''' | '''Make 20% weight by volume glucose solution''' | ||
| - | Add 20g powdered glucose to | + | Add 20g powdered glucose to 20 ml distilled water. Make up overall volume to 100 ml by adding more distilled water. Dissolve by using magnetic stirrer. |
| - | Filter sterilise the solution and store in 2 | + | Filter sterilise the solution and store in 2 50 ml Falcon tube. |
Revision as of 10:30, 16 July 2013
Learn how to make LB broth and agar
For one litre of broth we need...
- 10 g tryptone
- 5 g yeast extract
- 5 g NaCl
These were weighed out using a standard tabletop scale, accurate to 2 decimal places.
Add to ~800 ml of distilled water and mix using a magnetic stirrer. When dissolved, add more distilled water to bring the overall amount to 1000 ml (1 litre)
Pour into 200 ml Duran bottles, and add 3g of agar powder to each. This will not dissolve until the bottle is autoclaved for 20 minutes at 120oC.
We also made several 50 ml batches of LB broth, stored in conical flasks.
Learn how to make SOB growth media
For one litre of SOB we need...
- 5 g yeast extract
-20 g tryptone
- 0.584 g NaCl
- 0.186 g KCl
- 2.4 g MgSO4
The tryptone, yeast extract and MgSO4 were weighed out using a standard tabletop scale, accurate to 2 decimal places. The NaCl and KCl were weighed out using a scale accurate to 4 decimal places.
Dissolve into ~800 ml of distilled water using a magnetic stirrer, then add more distilled water until the overall volume is 1 litre.
Make 20% weight by volume glucose solution
Add 20g powdered glucose to 20 ml distilled water. Make up overall volume to 100 ml by adding more distilled water. Dissolve by using magnetic stirrer.
Filter sterilise the solution and store in 2 50 ml Falcon tube.
