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Revision as of 13:28, 22 October 2013

Contents 1 30-09 - 06-10-13 1.1 Generation of DelH Plasmid pHM04 15-09 1.1.1 Colony-PCR Conditions CP.W23.A 1.1.2 Result

30-09 - 06-10-13

Generation of DelH Plasmid pHM04 15-09

Colony-PCR Conditions CP.W23.A

Template	23x 1 picked colony, positive control
Expected length [bp]	663
Named	1A - 12H
Primer fw 10 µM	100x 2 µl VF2
Primer rev 10 µM	100x 2 µl DN07
iTaq Polymerase (2x)	100x 10 µl
ddH2O	96x 6 µl

Cycles	Temperature DelH [°C]	Time [s]
1	95	120
12	95	30
	68 (touchdown -0.5°C)	30
	72	45
18	95	30
	65	30
	72	45
1	12	inf

Result

Expected band: 663 bp
[[|200px|thumb|right|Fig.21.1 Colony PCR of Gibson assembled DelH-BB without mRFP 23 colonies]]