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Team:Penn/MaGellinToolbox
From 2013.igem.org
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<li>The Automation: a unique software package that predicts experimental outcomes and analyzes gel electrophoresis images to measure methylase functionality</li> | <li>The Automation: a unique software package that predicts experimental outcomes and analyzes gel electrophoresis images to measure methylase functionality</li> | ||
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| + | <div style="margin-left:auto;margin-right:auto;text-align:center"><figure><img border="0" src="https://static.igem.org/mediawiki/2013/4/4b/Penn_Toolbox.png" alt="Toolbox" width="700" height="395"><figcaption><i>Toolbox with the three essential components.</i></figcaption></figure></div> | ||
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Our toolbox satisfies 6 requirements: | Our toolbox satisfies 6 requirements: | ||
<ol> | <ol> | ||
Revision as of 08:59, 27 October 2013
Toolbox
- The Tool: a fusion between a DNA binding domain and a methylase enzyme
- The Assay: a digestion based assay, called MaGellin, to measure both methylase activity and sequence specificity
- The Automation: a unique software package that predicts experimental outcomes and analyzes gel electrophoresis images to measure methylase functionality
- Robust – includes functional fusion proteins for comparison
- Standardized – all inclusive in one-plasmid with simple multiple cloning sites, standard bisulfite sequencing primers, and a digestion based methylation assay built in
- Easy to Use – we programmed a software package to predict experimental outcomes and automate gel electrophoresis analysis
- Inexpensive – methylase activity and specificity can be screened by simply digesting and running a gel
- Noiseless – the bacterial chassis has no background CpG methylation
- Open Source – we BioBrick’ed the full plasmid and the methylases
