Team:Penn/MethylaseCharacterization
From 2013.igem.org
(Difference between revisions)
Line 68: | Line 68: | ||
<div style="margin-left:auto;margin-right:auto;text-align:center"><figure><img border="0" src="https://static.igem.org/mediawiki/2013/thumb/8/8c/3dplot-Converted.png/723px-3dplot-Converted.png" alt="Workflow" width="600px"><figcaption><i>Figure 4: The TALE-M.SssI with and without the TALE binding site present was induced with 0, .1, 1, and 2 mM of IPTG for 0, 2, 6, and 24 hours to find optimal expression conditions. Representative bands’ intensities were quantified. The targeting score (formula below) increases with site-specificity and decreases with enzymatic over activity as measured by the TALE without the binding site. </i></figcaption></figure></div> | <div style="margin-left:auto;margin-right:auto;text-align:center"><figure><img border="0" src="https://static.igem.org/mediawiki/2013/thumb/8/8c/3dplot-Converted.png/723px-3dplot-Converted.png" alt="Workflow" width="600px"><figcaption><i>Figure 4: The TALE-M.SssI with and without the TALE binding site present was induced with 0, .1, 1, and 2 mM of IPTG for 0, 2, 6, and 24 hours to find optimal expression conditions. Representative bands’ intensities were quantified. The targeting score (formula below) increases with site-specificity and decreases with enzymatic over activity as measured by the TALE without the binding site. </i></figcaption></figure></div> | ||
</br></br> | </br></br> | ||
- | + | P = TALE Binding Site</br> | |
M = No TALE Binding Site</br> | M = No TALE Binding Site</br> | ||
N = On Target Methylation Band</br> | N = On Target Methylation Band</br> | ||
F = Off Target Methylation Band</br></i> | F = Off Target Methylation Band</br></i> | ||
- | <div style="margin-left:auto;margin-right:auto;text-align:center"><figure><img border="0" src="https://static.igem.org/mediawiki/2013/f/fa/CodeCogsEqn42314.gif" alt="Workflow" width=" | + | <div style="margin-left:auto;margin-right:auto;text-align:center"><figure><img border="0" src="https://static.igem.org/mediawiki/2013/f/fa/CodeCogsEqn42314.gif" alt="Workflow" width="250px"><figcaption><i> </i></figcaption></figure></div> |
</br></br> | </br></br> | ||
Revision as of 11:58, 27 October 2013