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Team:Groningen/Labwork/17 July 2013
From 2013.igem.org
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<br> Made -80 C stock of the Munich backbone BBa_K823023, in tray iGEM 2012 A, position 26. | <br> Made -80 C stock of the Munich backbone BBa_K823023, in tray iGEM 2012 A, position 26. | ||
| - | <br> Inoculated the BBa_818000, made 5 tubes | + | <br> Inoculated the BBa_818000, made 5 tubes. Cells are to grow overnight in a 37 C shaker. |
<table border = "1"> | <table border = "1"> | ||
Revision as of 14:43, 17 July 2013
Mirjam
Check of the restriction done yesterday. This revealed that 5/7 colonies did contain the transformant. The cells of the fourth plate started to grow, so a mini prep analysis is performed. A restriction analysis is done with EcoRV.
A second confirmation of the constructs is done using PCR.
Inne
The E. coli cells with the BBa_k818000 that were to grow overnight didn't grow.
The tubes that were supposed to be turbid were still clear, however the negative control was also clear so that one did succeed.
Made -80 C stock of the Munich backbone BBa_K823023, in tray iGEM 2012 A, position 26.
Inoculated the BBa_818000, made 5 tubes. Cells are to grow overnight in a 37 C shaker.
| Sample | Composition |
| 1 | 4mL LB + 8uL Amp |
| 2 | 4mL LB + 8uL Amp |
| 3 | 4mL LB + 8uL Amp (negative control) |
| 4 | 4mL LB |
| 5 | 4mL LB (negative control) |
