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Team:DTU-Denmark/Notebook/17 July 2013
From 2013.igem.org
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- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp | - gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp | ||
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| + | ==Results== | ||
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| + | 1% agarose gel of today's PCR | ||
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| + | [[File:2013-07-17 his construct.jpg| 200px]] | ||
Revision as of 15:21, 18 July 2013
Contents |
208
Main purpose
- Miniprep
Who was in the lab
Gosia, Henrike
Procedure
- miniprep of RFP in pZA21 and cycAX in pZA21
- made gycerol stocks from ON cultures supposedly containing RFP in pZA21 and cycAX in pZA21
- gel of yesterday's PCR (to get AMO with USER endings) -> got the fragment
- purified AMO for USER from gel
- PCR to add His-tags to construct 1 (Sec-GFP-RFP) and construct 2 (TAT-GFP-RFP)
- gel on today's PCR -> construct 1 has no product, construct 2 has an unkown product with length 900 bp
Results
1% agarose gel of today's PCR
