Team:DTU-Denmark/Notebook/18 July 2013

From 2013.igem.org

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(Procedure)
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- made gel for yesterday's PCR of Nir operon -> empty
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* made gel for yesterday's PCR of Nir operon -> empty
-
- USER reaction with HAO + pZA21 and AMO + pZA21
+
* USER reaction with HAO + pZA21 and AMO + pZA21
-
- nandrop measurement of yesterday's miniprep showed it had very small yield, so we will redo it  
+
* nandrop measurement of yesterday's miniprep showed it had very small yield, so we will redo it  
-
- inocculated cultures for miniprep of RFP in pZA21 and cycAX in pZA21
+
* inocculated cultures for miniprep of RFP in pZA21 and cycAX in pZA21
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 +
=== Gel electrophoresis - Nir,

Revision as of 15:33, 18 July 2013


Contents

208


Main purpose


  • USER reaction

Who was in the lab


Gosia, Henrike, Julia

Procedure


  • made gel for yesterday's PCR of Nir operon -> empty
  • USER reaction with HAO + pZA21 and AMO + pZA21
  • nandrop measurement of yesterday's miniprep showed it had very small yield, so we will redo it
  • inocculated cultures for miniprep of RFP in pZA21 and cycAX in pZA21

=== Gel electrophoresis - Nir,