Team:UC-Santa Cruz/Project

From 2013.igem.org

(Difference between revisions)
(Overall project)
(The Experiments)
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=== The Experiments ===
=== The Experiments ===
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-Transfect caulobacter with florescent fusion protein plasmids.
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-Induce transformed caulobacter to express florescence.
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-Grow monolayer of caulobacter biofilm.
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- create tag florescent fusion protien plasmids,transfect into caulobacter, induce, check for polarized florescence.
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- create pumps/channels/pores tag fusion protien plasmids,transfect into caulobacter, induce, check for polarized florescence.
=== Part 3 ===
=== Part 3 ===

Revision as of 20:52, 9 August 2013


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Abstract

to save water and make cool bacteria


The world's six billion people are appropriating 54 percent of all the accessible freshwater contained in rivers, lakes and underground aquifers. - See more at: http://www.unwater.org/statistics.html#sthash.JfpKTp6p.dpuf

The total volume of water on Earth is about 1.4 billion km3. The volume of freshwater resources is around 35 million km3, or about 2.5 percent of the total volume. - See more at: http://www.unwater.org/statistics.html#sthash.JfpKTp6p.dpuf

To access this saltwater to accommodate the needs of the global population, many desalination techniques have been developed. The end goal of our project is a bacterial system to directly desalinate seawater using sunlight. The project also aims to provide a scaffold for bacterial manipulation of ionic solutions (ex. heavy metal bioremediation) for other groups.

building a monolayer biofilm to act as a selective membrane. Uses rhodopsins to pump ions in seawater.

Contents

Project Details

Part 2

The Experiments

-Transfect caulobacter with florescent fusion protein plasmids. -Induce transformed caulobacter to express florescence. -Grow monolayer of caulobacter biofilm. - create tag florescent fusion protien plasmids,transfect into caulobacter, induce, check for polarized florescence. - create pumps/channels/pores tag fusion protien plasmids,transfect into caulobacter, induce, check for polarized florescence.

Part 3

Results