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Team:DTU-Denmark/Notebook/23 July 2013
From 2013.igem.org
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==colony PCR gel== | ==colony PCR gel== | ||
[[File:2013-07-23 colony cyc rfp.jpg|600px]] | [[File:2013-07-23 colony cyc rfp.jpg|600px]] | ||
Revision as of 09:28, 24 July 2013
Results
Verification gel
1% gel to verify our earlier PCR purifications
wells (in bracets position of the purification in the gene box):
- 1: 1kb plus ladder
- 2: HAO extracted from N. europeae with flanking primers (A4)
- 3: AMO extracted from N. europeae with flanking primers (A6)
- 4: cycAX extracted from N. europeae with flanking primers (A5)
- 5: Nir extracted from P. aeruginosa with flanking primers (A7)
- 6: Nir extracted from P. aeruginosa with flanking primers (A8)
- 7: Nir extracted from P. aeruginosa with flanking primers (B8)
- 8: Nir extracted from P. aeruginosa with flanking primers (B9)
- 9: pZA21 USER fragment, DpnI treated (F1)
- 10: cycAX USER fragment (G1)
- 11: AMO USER fragment (G2)
- 12: HAO USER fragment (G3)
- 13: Nir USER fragment (G4)
- 14: AMO USER fragment (G5)
- 15: 1kb plus ladder
colony PCR gel
