Team:Groningen/protocols/PCR

From 2013.igem.org

(Difference between revisions)
Line 28: Line 28:
<li>PCR tubes</li>
<li>PCR tubes</li>
<li>F- and R-primer</li>
<li>F- and R-primer</li>
 +
<li>DNTPs</li>
 +
<li>Phusion buffer</li>
<li>H<sub>2</sub>O</li>
<li>H<sub>2</sub>O</li>
<li>Phusion Polymerase</li>
<li>Phusion Polymerase</li>

Revision as of 08:46, 26 July 2013

PCR


Materials:
  • PCR tubes
  • F- and R-primer
  • DNTPs
  • Phusion buffer
  • H2O
  • Phusion Polymerase

Steps: Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf