Team:Groningen/protocols/PCR

From 2013.igem.org

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Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf
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<br>Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf
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Revision as of 08:48, 26 July 2013

PCR


Materials:
  • PCR tubes
  • F- and R-primer
  • DNTPs
  • Phusion buffer
  • H2O
  • Phusion Polymerase
  • DNA template

Steps:
Cycling instructions:
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf