Team:Groningen/protocols/GelElectrophoresis

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(Difference between revisions)
Line 29: Line 29:
<li>0.8 or 1.5% agarose gel</li>
<li>0.8 or 1.5% agarose gel</li>
<li>Gel tray</li>
<li>Gel tray</li>
 +
<li>2x Loading Dye
<li>DNA samples</li>
<li>DNA samples</li>
<li>DNA 1kb GeneRuler of Fermentas</li>
<li>DNA 1kb GeneRuler of Fermentas</li>

Revision as of 10:26, 28 July 2013

Gel electrophoresis

Materials:

  • Power supply
  • 0.8 or 1.5% agarose gel
  • Gel tray
  • 2x Loading Dye
  • DNA samples
  • DNA 1kb GeneRuler of Fermentas

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 2x.
The samples are loaded on a 0.8% or 1.5% agarose gel (diluted in TBE buffer 1x), with next to it a DNA 1kb GeneRuler of Fermentas.
The gel is placed in TBE buffer 1x and a 90V electric potential is applied to the gel for 24-45 minutes to seperate the bands.