Team:Waterloo

From 2013.igem.org

(Difference between revisions)
(Cropped and resized photos. All headshots present.)
(Link formatting and navbar colour changes. Fetching notebook from an uploaded file now and dynamically generating sublinks.)
Line 3: Line 3:
     TODO:
     TODO:
     - The header takes too much space - especially on the home page
     - The header takes too much space - especially on the home page
-
    - Make photos lower res!
 
   -->
   -->
-
 
   <head>
   <head>
     <!--<script type="text/javascript" src="https://2013.igem.org/common/jquery-latest.min.js"></script>-->
     <!--<script type="text/javascript" src="https://2013.igem.org/common/jquery-latest.min.js"></script>-->
Line 101: Line 99:
         color: white;
         color: white;
-
         /* http://www.colorzilla.com/gradient-editor/#006e2e+0,00e059+100;Custom */
+
         /* http://www.colorzilla.com/gradient-editor/#5abfce+0,0ba5bc+100;Custom */
-
         background: rgb(0,110,46); /* Old browsers */
+
         background: rgb(90,191,206); /* Old browsers */
-
         background: -moz-linear-gradient(45deg,  rgba(0,110,46,1) 0%, rgba(0,224,89,1) 100%); /* FF3.6+ */
+
         background: -moz-linear-gradient(45deg,  rgba(90,191,206,1) 0%, rgba(11,165,188,1) 100%); /* FF3.6+ */
-
         background: -webkit-gradient(linear, left bottom, right top, color-stop(0%,rgba(0,110,46,1)), color-stop(100%,rgba(0,224,89,1))); /* Chrome,Safari4+ */
+
         background: -webkit-gradient(linear, left bottom, right top, color-stop(0%,rgba(90,191,206,1)), color-stop(100%,rgba(11,165,188,1))); /* Chrome,Safari4+ */
-
         background: -webkit-linear-gradient(45deg,  rgba(0,110,46,1) 0%,rgba(0,224,89,1) 100%); /* Chrome10+,Safari5.1+ */
+
         background: -webkit-linear-gradient(45deg,  rgba(90,191,206,1) 0%,rgba(11,165,188,1) 100%); /* Chrome10+,Safari5.1+ */
-
         background: -o-linear-gradient(45deg,  rgba(0,110,46,1) 0%,rgba(0,224,89,1) 100%); /* Opera 11.10+ */
+
         background: -o-linear-gradient(45deg,  rgba(90,191,206,1) 0%,rgba(11,165,188,1) 100%); /* Opera 11.10+ */
-
         background: -ms-linear-gradient(45deg,  rgba(0,110,46,1) 0%,rgba(0,224,89,1) 100%); /* IE10+ */
+
         background: -ms-linear-gradient(45deg,  rgba(90,191,206,1) 0%,rgba(11,165,188,1) 100%); /* IE10+ */
-
         background: linear-gradient(45deg,  rgba(0,110,46,1) 0%,rgba(0,224,89,1) 100%); /* W3C */
+
         background: linear-gradient(45deg,  rgba(90,191,206,1) 0%,rgba(11,165,188,1) 100%); /* W3C */
-
         filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#006e2e', endColorstr='#00e059',GradientType=1 ); /* IE6-9 fallback on horizontal gradient */
+
         filter: progid:DXImageTransform.Microsoft.gradient( startColorstr='#5abfce', endColorstr='#0ba5bc',GradientType=1 ); /* IE6-9 fallback on horizontal gradient */
       }
       }
Line 173: Line 171:
       .link {
       .link {
         font-size: 24px;
         font-size: 24px;
 +
        line-height: 25px;
       }
       }
       .sublink {
       .sublink {
-
         margin-left: 20px;
+
         margin: 0 0 14px 20px;
         font-size: 20px;
         font-size: 20px;
 +
        line-height: 23px;
       }
       }
       /* TODO animate resizing this */
       /* TODO animate resizing this */
Line 220: Line 220:
     '#/Home',
     '#/Home',
     '#/Project',
     '#/Project',
-
    '#/Team',
 
     '#/MathModelling',
     '#/MathModelling',
-
     '#/Outreach',
+
     '#/HumanPractices',
     '#/Gallery',
     '#/Gallery',
     '#/Notebook',
     '#/Notebook',
     '#/Safety',
     '#/Safety',
-
     '#/Sponsors'
+
     '#/Sponsors',
 +
    '#/Team'
   ];
   ];
 +
  var MONTHS = {
 +
    'january': 'January',
 +
    'february': 'February',
 +
    'march': 'March',
 +
    'april': 'April',
 +
    'may': 'May',
 +
    'june': 'June',
 +
    'july': 'July',
 +
    'august': 'August',
 +
    'september': 'September',
 +
    'october': 'October',
 +
    'november': 'November',
 +
    'december': 'December'
 +
  };
   var navBar = null;
   var navBar = null;
   var page_idx_map = {};
   var page_idx_map = {};
Line 292: Line 306:
     for (var i = 0; i < this.links.length; i++) {
     for (var i = 0; i < this.links.length; i++) {
       var link_wrapper = $('<li class="hiddenSublinks"/>');
       var link_wrapper = $('<li class="hiddenSublinks"/>');
-
       var link_string = '<div class="link"><a href="{0}">- {1}</a></div>';
+
       var link_string = '<div class="link"><a href="{0}">{1}</a></div>';
       var link = $(link_string.format(this.links[i].link, this.links[i].text));
       var link = $(link_string.format(this.links[i].link, this.links[i].text));
       link_wrapper.append(link);
       link_wrapper.append(link);
Line 298: Line 312:
       var sublinks = this.sublinks[i];
       var sublinks = this.sublinks[i];
       for (var j = 0; j < sublinks.length; j++) {
       for (var j = 0; j < sublinks.length; j++) {
-
         var sublink_string = '<div class="sublink"><a href="{0}">- {1}</a></div>';
+
         var sublink_string = '<div class="sublink"><a href="{0}">{1}</a></div>';
         var sublink = $(sublink_string.format(sublinks[j].link, sublinks[j].text));
         var sublink = $(sublink_string.format(sublinks[j].link, sublinks[j].text));
         link_wrapper.append(sublink);
         link_wrapper.append(sublink);
Line 310: Line 324:
     return page_links;
     return page_links;
   };
   };
 +
 +
 +
  function createNavBar(notebook_sublinks, show_notebook) {
 +
    // Create NavBar
 +
    navBar = new NavBar();
 +
    //navBar.addLink(new Link('#/Home', 'Home'));
 +
    navBar.addLink(
 +
      new Link('#/Project', 'Project'),
 +
      [
 +
        new Link('#/Project/Abstract', 'Abstract'),
 +
        new Link('#/Project/Video', 'Video'),
 +
        new Link('#/Project/Results', 'Results'),
 +
        new Link('#/Project/Future', 'Future Aspirations'),
 +
        new Link('#/Project/Biobricks', 'Biobricks')
 +
      ]
 +
    );
 +
    navBar.addLink(
 +
      new Link('#/MathModelling', 'Math & Modelling'),
 +
      [
 +
        new Link('#/MathModelling/Switch', 'Switch Modelling'),
 +
        new Link('#/MathModelling/PopulationInfection', 'Population and Infection Modelling'),
 +
        new Link('#/MathModelling/PhageParticle', 'Phage Particle Production Modelling')
 +
      ]
 +
    );
 +
    navBar.addLink(
 +
      new Link('#/HumanPractices', 'HumanPractices'),
 +
      [
 +
        new Link('#/HumanPractices/IntentToInvent', 'Intent to Invent'),
 +
        new Link('#/HumanPractices/Vlogs', 'Vlogs'),
 +
        new Link('#/HumanPractices/SpecialProject', 'Special Project')
 +
      ]
 +
    );
 +
    navBar.addLink(
 +
      new Link('#/Gallery', 'Gallery'),
 +
      [
 +
        new Link('#/Gallery/IntentToInvent', 'Intent to Invent'),
 +
        new Link('#/Gallery/Laboratory', 'Laboratory')
 +
      ]
 +
    );
 +
    if (show_notebook) {
 +
      navBar.addLink(
 +
        new Link('#/Notebook', 'Notebook'),
 +
        notebook_sublinks
 +
      );
 +
    }
 +
    navBar.addLink(
 +
      new Link('#/Safety', 'Safety')
 +
    );
 +
    navBar.addLink(
 +
      new Link('#/Sponsors', 'Sponsors'),
 +
      [
 +
        new Link('#/Sponsors/Sponsors', 'Sponsors')
 +
      ]
 +
    );
 +
    navBar.addLink(
 +
      new Link('#/Team', 'Team'),
 +
      [
 +
        new Link('#/Team/Administrators', 'Administrators'),
 +
        new Link('#/Team/Advisors', 'Advisors'),
 +
        new Link('#/Team/LabDesign', 'Lab & Design'),
 +
        new Link('#/Team/HumanPractices', 'Human Practices'),
 +
        new Link('#/Team/MathModelling', 'Math & Modelling')
 +
      ]
 +
    );
 +
    var nav_bar_element = $('.navBar');
 +
    nav_bar_element.append(navBar.render());
 +
    nav_bar_element.append(socialButtons(
 +
      [
 +
        new ImageLink('https://www.facebook.com/WaterlooiGEM', 'https://static.igem.org/mediawiki/2013/8/81/Waterloo_facebook_button_64.jpg'),
 +
        new ImageLink('http://www.youtube.com/user/WaterlooiGEM?feature=watch', 'https://static.igem.org/mediawiki/2013/8/8f/Waterloo_youtube_button_64.jpg'),
 +
        new ImageLink('https://twitter.com/Waterloo_iGEM', 'https://static.igem.org/mediawiki/2013/a/a9/Waterloo_twitter_button_64.jpg'),
 +
      ]
 +
    ));
 +
  }
   function socialButtons(image_links) {
   function socialButtons(image_links) {
Line 354: Line 442:
       }
       }
     );
     );
-
     setupGroup('#teamPhotos',
+
     setupGroup('#labTeamPhotos',
       {
       {
         'Paul': 'https://static.igem.org/mediawiki/2013/b/bd/Waterloo_paul.JPG',
         'Paul': 'https://static.igem.org/mediawiki/2013/b/bd/Waterloo_paul.JPG',
Line 370: Line 458:
       }
       }
     );
     );
 +
    setupGroup('#mathModellingTeamPhotos', {});
 +
    setupGroup('#humanPracticesTeamPhotos', {});
     // TODO
     // TODO
     setupGroup('#intentToInventPhotos', {});
     setupGroup('#intentToInventPhotos', {});
     setupGroup('#labPhotos', {});
     setupGroup('#labPhotos', {});
 +
  }
 +
 +
  /**
 +
  * Renders raw notebook text into lists on the #notebook element.
 +
  * Returns notebook sublinks that should be rendered in the navbar.
 +
  */
 +
  function createNotebook(raw) {
 +
    var sublinks = [];
 +
    var notebook_elem = $('#notebook');
 +
    var current_list = null;
 +
    var current_month = null;
 +
 +
    var lines = raw.split('\n');
 +
    for (var i = 0; i < lines.length; i++) {
 +
      var line = lines[i].trim();
 +
      if (line == '') continue;
 +
      var maybe_month_lower = line.substring(0, line.indexOf(' ')).toLowerCase();
 +
      var maybe_month = MONTHS[maybe_month_lower];
 +
      if (maybe_month) {
 +
        if (current_month != maybe_month) {
 +
          current_month = maybe_month;
 +
          sublinks.push(new Link('#/Notebook/' + current_month, current_month));
 +
          notebook_elem.append($('<h1 id="{0}">{1}</h1>'.format('s_Notebook_' + current_month, current_month)));
 +
          current_list = $('<ul></ul>');
 +
          notebook_elem.append(current_list);
 +
        }
 +
        if (!current_list) {
 +
          current_list = $('<ul></ul>');
 +
          notebook_elem.append(current_list);
 +
        }
 +
        current_list.append($('<li>{0}</li>'.format(line)));
 +
      }
 +
    }
 +
    return sublinks;
   }
   }
Line 385: Line 509:
       var new_left_layer = new_left * layer_factors[layer] + LAYER_OFFSET;
       var new_left_layer = new_left * layer_factors[layer] + LAYER_OFFSET;
-
      //TODO TEMP - remove once photos are smaller
 
       /*
       /*
-
       if (idx == 2 || page_idx == 2) {
+
      // Remove animation from team page
 +
       if (idx == 8 || page_idx == 8) {
         $('#layer' + layer).css('left', new_left_layer + 'px');
         $('#layer' + layer).css('left', new_left_layer + 'px');
         if (complete) complete();
         if (complete) complete();
Line 456: Line 580:
           //page.scrollTop(scroll_dest);
           //page.scrollTop(scroll_dest);
-
           page.animate({scrollTop: scroll_dest},  
+
           page.animate({scrollTop: scroll_dest},
             {
             {
               'duration': 400,
               'duration': 400,
Line 496: Line 620:
     }
     }
-
     // Create NavBar
+
     // Fetch notebook data and create section
-
     navBar = new NavBar();
+
     var notebook_url = 'https://static.igem.org/mediawiki/2013/d/dc/Waterloo_notebook.txt';
-
    //navBar.addLink(new Link('#/Home', 'Home'));
+
     $.ajax({
-
    navBar.addLink(
+
       url: notebook_url,
-
      new Link('#/Project', 'Project'),
+
       type: 'GET',
-
      [
+
       success: function(data) {
-
        new Link('#/Project/Abstract', 'Abstract'),
+
         var notebook_sublinks = createNotebook(data);
-
        new Link('#/Project/Video', 'Video'),
+
         createNavBar(notebook_sublinks, true);
-
        new Link('#/Project/Results', 'Results'),
+
       },
-
        new Link('#/Project/Future', 'Future Aspirations'),
+
       error: function() {
-
        new Link('#/Project/Biobricks', 'Biobricks')
+
         // On failure create the navbar anyway.
-
      ]
+
         createNavBar([], false);
-
    );
+
       }
-
     navBar.addLink(
+
     });
-
       new Link('#/Team', 'Team'),
+
-
       [
+
-
        new Link('#/Team/Administrators', 'Administrators'),
+
-
        new Link('#/Team/LabDesign', 'Lab & Design')
+
-
       ]
+
-
    );
+
-
    navBar.addLink(
+
-
      new Link('#/MathModelling', 'Math & Modelling'),
+
-
      [
+
-
         new Link('#/MathModelling/Switch', 'Switch Modelling'),
+
-
         new Link('#/MathModelling/PopulationInfection', 'Population and Infection Modelling'),
+
-
        new Link('#/MathModelling/PhageParticle', 'Phage Particle Production Modelling')
+
-
      ]
+
-
    );
+
-
    navBar.addLink(
+
-
       new Link('#/Outreach', 'Outreach'),
+
-
       [
+
-
        new Link('#/Outreach/IntentToInvent', 'Intent to Invent'),
+
-
         new Link('#/Outreach/Vlogs', 'Vlogs')
+
-
      ]
+
-
    );
+
-
    navBar.addLink(
+
-
      new Link('#/Gallery', 'Gallery'),
+
-
      [
+
-
         new Link('#/Gallery/IntentToInvent', 'Intent to Invent'),
+
-
        new Link('#/Gallery/Laboratory', 'Laboratory')
+
-
      ]
+
-
    );
+
-
    navBar.addLink(
+
-
      new Link('#/Notebook', 'Notebook'),
+
-
      [
+
-
        // TODO - Months
+
-
      ]
+
-
    );
+
-
    navBar.addLink(
+
-
      new Link('#/Safety', 'Safety')
+
-
    );
+
-
    navBar.addLink(
+
-
       new Link('#/Sponsors', 'Sponsors'),
+
-
      [
+
-
        new Link('#/Sponsors/Advisors', 'Advisors'),
+
-
        new Link('#/Sponsors/Sponsors', 'Sponsors')
+
-
      ]
+
-
     );
+
-
    var nav_bar_element = $('.navBar');
+
-
    nav_bar_element.append(navBar.render());
+
-
    nav_bar_element.append(socialButtons(
+
-
      [
+
-
        new ImageLink('https://www.facebook.com/WaterlooiGEM', 'https://static.igem.org/mediawiki/2013/8/81/Waterloo_facebook_button_64.jpg'),
+
-
        new ImageLink('http://www.youtube.com/user/WaterlooiGEM?feature=watch', 'https://static.igem.org/mediawiki/2013/8/8f/Waterloo_youtube_button_64.jpg'),
+
-
        new ImageLink('https://twitter.com/Waterloo_iGEM', 'https://static.igem.org/mediawiki/2013/a/a9/Waterloo_twitter_button_64.jpg'),
+
-
      ]
+
-
    ));
+
     setupPhotos();
     setupPhotos();
Line 632: Line 703:
           <h1 id="s_Project_Future">Future Aspirations</h1>
           <h1 id="s_Project_Future">Future Aspirations</h1>
           <h1 id="s_Project_Biobricks">Biobricks</h1>
           <h1 id="s_Project_Biobricks">Biobricks</h1>
-
        </div>
 
-
      </div>
 
-
 
-
      <!--Team-->
 
-
      <div id="p2" class="page">
 
-
        <div class="pageStuff">
 
-
          <h1 id="s_Team_Administrators">Administrators</h1>
 
-
          <div id="administratorPhotos" class="photoSection"></div>
 
-
          <div class="clearFix"></div>
 
-
          <h1 id="s_Team_LabDesign">Lab &amp; Design</h1>
 
-
          <div id="teamPhotos" class="photoSection"></div>
 
         </div>
         </div>
       </div>
       </div>
Line 648: Line 708:
       <!--Math & Modelling-->
       <!--Math & Modelling-->
-
       <div id="p3" class="page">
+
       <div id="p2" class="page">
         <div class="pageStuff">
         <div class="pageStuff">
           <h1 id="s_MathModelling_Switch">Switch Modelling</h1>
           <h1 id="s_MathModelling_Switch">Switch Modelling</h1>
Line 658: Line 718:
             a b c
             a b c
           </p>
           </p>
-
           <h1 id="s_MathModelling_PhageParticle">Phage Particle Production Modeling</h1>
+
           <h1 id="s_MathModelling_PhageParticle">Phage Particle Production Modelling</h1>
           <p>
           <p>
             a b c
             a b c
Line 665: Line 725:
       </div>
       </div>
-
       <!--Outreach-->
+
       <!--HumanPractices-->
-
       <div id="p4" class="page">
+
       <div id="p3" class="page">
         <div class="pageStuff">
         <div class="pageStuff">
-
           <h1 id="s_Outreach_IntentToInvent">Intent to Invent</h1>
+
           <h1 id="s_HumanPractices_IntentToInvent">Intent to Invent</h1>
           <p>
           <p>
             <!-- TODO video? -->
             <!-- TODO video? -->
           </p>
           </p>
-
           <h1 id="s_Outreach_Vlogs">Vlogs</h1>
+
           <h1 id="s_HumanPractices_Vlogs">Vlogs</h1>
 +
          <h1 id="s_HumanPractices_SpecialProject">Special Project</h1>
         </div>
         </div>
       </div>
       </div>
       <!--Gallery-->
       <!--Gallery-->
-
       <div id="p5" class="page">
+
       <div id="p4" class="page">
         <div class="pageStuff">
         <div class="pageStuff">
           <h1 id="s_Gallery_IntentToInvent">Intent to Invent</h1>
           <h1 id="s_Gallery_IntentToInvent">Intent to Invent</h1>
Line 691: Line 752:
       <!--Notebook-->
       <!--Notebook-->
-
       <div id="p6" class="page">
+
       <div id="p5" class="page">
         <div class="pageStuff">
         <div class="pageStuff">
-
           <div id="notebook">
+
           <div id="notebook"> </div>
-
            <!-- TODO -->
+
-
            <ul>
+
-
              <li>March 13th, 2013: Made stocks of 6 oligos which will be annealed to make the PhiC31 attP site.</li>
+
-
              <li>March 18th, 2013: Inoculated strain #118 from frozen stock.</li>
+
-
              <li>March 19th, 2013: The cultures from yesterday did not grow. Streak out strain #118 onto regular LB and Cm + LB to check for contamination of stock. </li>
+
-
              <li>March 20th, 2013: There were multiple morphologies of colonies on the Cm + LB plate from yesterday therefore the frozen stock is likely contaminated.</li>
+
-
              <li>March 21st, 2013: Make new frozen stock of DH5-alpha carrying pSB1C3 + RFP stuffer fragment (strain #151).
+
-
              </li>
+
-
              <li>March 22nd, 2013: Maxiprep pSB1C3 from strain #151. </li>
+
-
              <li>March 26th, 2013: Restriction digest of pSB1C3 with EcoRI and PstI showed a smear on the gel. </li>
+
-
              <li>March 27th, 2013: Restriction digest of pSB1C3 from maxi-prep showed genomic DNA contamination. We were able to gel extract pSB1C3 from the gel to separate it from genomic DNA contamination. Transform DH5-alpha with gel extracted pSB1C3. </li>
+
-
              <li>March 28th, 2013: Transform plate showed red colonies. Our competent cells work. </li>
+
-
              <li>April 1st, 2013: Inoculated strain #151. </li>
+
-
              <li>April 2nd, 2013: Hpdo is received as miniprep and agar stab. Streak out Hpdo on a LB + Km plate. Miniprep pSB1C3. Nanodrop received Hpdo but we got a bad concentration maybe because we do not know what buffer to blank with. The Hpdo streak plate grew. Store Hpdo streak plate in the fridge.</li>
+
-
              <li>April 3rd, 2013: Restriction digest of pSB1C3. Gel extract the linearized pSB1C3 cut with EcoRI and PstI. The gel extracted samples did not contain DNA so we discarded them. We are not sure what went wrong. Someone forgot to take a picture of the gel. Inoculate strain #151. </li>
+
-
              <li>April 4th, 2013: Miniprep pSB1C3. </li>
+
-
              <li>April 5th, 2013: Maxiprep Hpdo. This later turned out to be contaminated with genomic DNA. </li>
+
-
              <li>April 6th, 2013: Restriction digest of pSB1C3 shows genomic DNA contamination. Inoculate strain #151. </li>
+
-
              <li>April 7th, 2013: Miniprep pSB1C3 from strain #151.</li>
+
-
              <li>April 8th, 2013: Restriction digest of pSB1C3 with EcoRI and PstI. Gel extract linearized pSB1C3. </li>
+
-
              <li>April 10th, 2013: Ligation of 6 oligos to form the PhiC31 attP site into pSB1C3. </li>
+
-
              <li>April 11th, 2013: Diagnostic gel to check linearized pSB1C3 from April 10th, 2013. No gel red added no bands observed. </li>
+
-
              <li>April 12th, 2013: Transformation of ligated product from April 10th, 2013. No colonies were seen. At this point we are not sure why. </li>
+
-
              <li>April 16th, 2013: Restriction digest of pSB1C3 with EcoRI and PstI. Gel extract linearlized pSB1C3. </li>
+
-
              <li>April 17th, 2013: Diagnostic gel confirms gel extracted product from yesterday is linear and of the expected size. </li>
+
-
              <li>April 22nd, 2013: Ligation of 6 oligos to form the PhiC31 attP site into pSB1C3. </li>
+
-
              <li>April 23rd, 2013: Transformation of ligated product from April 22nd, 2013. No colonies were seen. At this point we are not sure why. </li>
+
-
              <li>April 24th, 2013: Transformation control using pSB1C3 confirms our competent DH5-alpha cells are still good. </li>
+
-
              <li>April 30th, 2013: Ligation to check ligase activity.</li>
+
-
              <li>April 31st, 2013: Transformation to check ligase activity. Inoculate culture of strain #152 containing Hpdo.</li>
+
-
              <li>May 1st, 2013: Miniprep Hpdo. Diagnostic gel confirms ligase is working correctly.</li>
+
-
              <li>May 6th, 2013: Making Amp + LB plates and streaking out E. coli carrying pSH62. pSH62 has the gene encoding PhiC31 integrase.  </li>
+
-
              <li>May 7th, 2013: Streak plate from yesterday looks good, store in fridge. Inoculate cultures from that streak plate. Diagnostic gel from gel extracted samples done on April 17th, 2013 show linearized pSB1C3 even though nanodrop values are negitive. Inoculate cultures of strain #151 and streak out a fresh plate of strain #151. </li>
+
-
              <li>May 8th, 2013: Inoculation from streak plate of E. coli carrying pSH62 did not grow. Inoculate again from the streak plate with fresh Terrific broth. Miniprep strain #151. Inoculate strain #152.  </li>
+
-
              <li>May 9th, 2013: Make frozen stock of DH5-alpha carrying pSH62 --> strain #153 and store in iGEM strain box 3. Miniprep strains #152 and #153. Restriction digest of pSB1C3 with EcoRI and PstI. The pSB1C3 looks good on the gel. Gel extract the samples. The nanodrop values of the gel extracted samples were bad. </li>
+
-
              <li>May 10th, 2013: Run a diagnostic gel with our gel extracted samples from yesterday. Though the nanodrop values are bad the linearlized backbone is clearly visible at the expected size on a diagnostic gel. To find an estimate of the concentrations we ran the gel extracted samples from yesterday on an agarose gel next to 100ng of lambda / HindIII ladder. This indicated the gel extracted samples had a concentration of ~ 5ng/uL. </li>
+
-
              <li>May 12th, 2013: Inoculate strains #154 and #155 containing pUC118 and pUC119. </li>
+
-
              <li>May 13th, 2013: Make frozen stock of strains #154 and #155. Miniprep strains #154 and #155. Amplify Hpdo minus gene VIII with KOD from maxiprep, Standford gift sample, May 1st miniprep and May 9th miniprep. </li>
+
-
              <li>May 14th, 2013: Gel extraction of Hpdo minus gene VIII. Run the gel extracted samples by a lambda / HindIII ladder to estimate the concentration.</li>
+
-
              <li>May 15th, 2013: Concentrate pSB1C3 cut with EcoRI and PstI with the speed vac. Self ligation of Hpdo minus gene VIII. Amplify PhiC31 integrase for pSH62. </li>
+
-
              <li>May 16th, 2013:      </li>
+
-
            </ul>
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Revision as of 04:09, 29 July 2013

Abstract

This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract. This is the abstract.

Video

Results

Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results Results.

Future Aspirations

Biobricks

Switch Modelling

x y z

Population & Infection Modelling

a b c

Phage Particle Production Modelling

a b c

Intent to Invent

Vlogs

Special Project

Intent to Invent

Laboratory

Safety

This page is still in progress

Sponsors

Administrators

Advisors

Lab & Design

Human Practices

Math & Modelling

 

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