Team:Groningen/Labwork/29 July 2013

From 2013.igem.org

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<h2>Friso</h2>
<h2>Friso</h2>
Made a restriction digestion for Spec purified.
Made a restriction digestion for Spec purified.
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<h2>Claudio</h2>
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<br>Two GFP reporters are picked from the iGEM database: BBa_E0840 and BBa_E0240 (backbone: pSB1C3). Those are transformed into <i>E. Coli</i> DH5&Alpha; (Cm resistance).
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<br>The plasmid containing mKate2 (red fluorescent protein into pGEM-T Easy vector) is inoculated in LB + Ampicillin resistance.
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Revision as of 11:14, 29 July 2013

Mirjam

Run the samples of the colony PCR of BBa_K823823 combined with the LacI promoter made on 27-07 on gel (order 1-8).
There are no bands around 1500 bp.

Run the purified PCR product of spec on gel.
The purified spec shows a nice band.

Run the PCR products of silk 2 on gel to do gel purification.
Only one of the tubes showed a band around the expected height.

Run the PCR products of silk 2 on gel to do gel purification.

Made a double restriction digestion with fast restriction digest for Pdes and CheY with respectively EcoRI and BamHI; and BamHI and PstI.

Chaline

Made a ligation for the knockout system of Des, with Des UP, Tet and Des Down

Friso

Made a restriction digestion for Spec purified.

Claudio


Two GFP reporters are picked from the iGEM database: BBa_E0840 and BBa_E0240 (backbone: pSB1C3). Those are transformed into E. Coli DH5Α (Cm resistance).
The plasmid containing mKate2 (red fluorescent protein into pGEM-T Easy vector) is inoculated in LB + Ampicillin resistance.