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Team:DTU-Denmark/Notebook/29 July 2013
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Names and numbers of colonies as well as plates names and primers are: | Names and numbers of colonies as well as plates names and primers are: | ||
| - | * HAO 1-6 in pZA21 USER, primers | + | * HAO 1-6 in pZA21 USER, primers 28a, 28b |
| - | * AMO 1-9 in pZA21 USER, primers | + | * AMO 1-9 in pZA21 USER, primers 29a, 29b |
| - | PCR programms based on [[Team:DTU-Denmark/Methods/PCR| standard PCR programm]] with | + | PCR programms based on [[Team:DTU-Denmark/Methods/PCR| standard PCR programm]] with annealing temperatures 52C for HAO and 56C for AMO and extension time 3:00 mins. |
==Results== | ==Results== | ||
Revision as of 12:08, 29 July 2013
29 July 2013
Contents |
lab 208
Main purpose
- Colony PCR from Pseudomonas aeruginosa (PAO) to isolate Nir.
- Colony PCR to check the presence of AMO and HAO in transormants E.coli.
Who was in the lab
Kristian, Gosia, Henrike, Julia
Procedure
Colony PCR
Colony PCR was performed according to standard method in order to check if transformed cells which grew on medium contain desired insert in pZA21 plasmid. The colonies were chosen from agar plate to be checked. Names and numbers of colonies as well as plates names and primers are:
- HAO 1-6 in pZA21 USER, primers 28a, 28b
- AMO 1-9 in pZA21 USER, primers 29a, 29b
PCR programms based on standard PCR programm with annealing temperatures 52C for HAO and 56C for AMO and extension time 3:00 mins.
Results
Conclusion
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