Team:Penn State/Cas9Project

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<h1 style="color: green"> Plant Promoter Project</h1>
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<h1 style="color: green"> Cas9 in Plants Project </h1>
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As plants are still novel organisms for most of synthetic biology, we we are interested in developing methods of control for our projects. Currently the Cauliflower Mosaic Virus 35S promoter is the most widely used plant promoter. In hopes of increasing the availability of plant promoters, our project aims at testing viral promoters due to their relative efficiency, as well as cytoskeletal protein promoters due to their natural abundance.  Testing these promoters in parallel with the CaMV 35S will create a plant promoter catalog which can be used for future iGEMers exploration of plant synthetic biology.  
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A CRISPR/cas9 system is a large protein guided by a self-guiding RNA, which is capable of targeting specific DNA sequences. Cas9 has been characterized previously in bacteria and mammalian cells. Often targeted to a promoter region, Cas9 acts as highly effective gene repressing tool. The goal of the cas9 project is to make this regulatory tool available to plant genetic engineering.
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Revision as of 19:44, 29 July 2013

Cas9 in Plants Project

A CRISPR/cas9 system is a large protein guided by a self-guiding RNA, which is capable of targeting specific DNA sequences. Cas9 has been characterized previously in bacteria and mammalian cells. Often targeted to a promoter region, Cas9 acts as highly effective gene repressing tool. The goal of the cas9 project is to make this regulatory tool available to plant genetic engineering.

Introduction

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Background

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Method

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Results

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Discussion

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Further Study

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