Team:TU-Delft/Notebook/2013/07/08/

From 2013.igem.org

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==8th of July==
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==7th of August==
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<p> 1. Biobricks from the iGEM registry were streaked on Agar plates.<br>
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<p> 1. Restriction digestion of pBAD dor pSB1C# backbone. <br>  
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2. Restreaking <i>Staphyllococcus delphini , Bacillus subtilis </i> and DH5-alpha cells. <br>
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2. Transformation of the pTET backbone with Lysis cassette, which was done on 5/7/13.<br>
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3. Analysis of the Gel electrophoresis in lab meeting. pBAD and pT7 was not that good. AIP sender and receiver also shows contamination of wells. <br>
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4. The biobricks: pBAD, pT7, GFP, AIP sender(Agr A: AgrC). AIP receiver(AgrB: AgrD), pTET:lysis were grown on Agar plates. But the negative control is not convincing, so Gel electrophoresis is run on random colonies from the plates to check. <br>
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Revision as of 10:02, 19 August 2013

Notebook

June
SunMonTueWedThuFriSat
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30
July
SunMonTueWedThuFriSat
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31










August
SunMonTueWedThuFriSat
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
September
SunMonTueWedThuFriSat
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30












7th of August


Lab work

1. Restriction digestion of pBAD dor pSB1C# backbone.
2. Restreaking Staphyllococcus delphini , Bacillus subtilis and DH5-alpha cells.

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