Team:Groningen/Labwork/1 August 2013

From 2013.igem.org

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<br>Made a restriction for Pdes with EcoRI and BamHI, for CheY with BamHI and PstI and for BBa_k823823 with EcoRI and PstI.
<br>Made a restriction for Pdes with EcoRI and BamHI, for CheY with BamHI and PstI and for BBa_k823823 with EcoRI and PstI.
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<p>
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<h2>Chaline</h2>
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<h2>Sebas</h2>
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<p>
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<h2>Sander</h2>

Revision as of 09:20, 1 August 2013

Mirjam

Plates for the transformation of ΔDes, ΔCheY and ΔDes/ΔCheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to E.coli. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
Inoculated the colonies into LB with ampicillin to perform a miniprep later on.
Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI. Did a gel purification to obtain the purified fragment.
Inoculated B.subtilis colonies for transformation.
Made a restriction for Pdes with EcoRI and BamHI, for CheY with BamHI and PstI and for BBa_k823823 with EcoRI and PstI.

Chaline

Sebas

Sander