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Team:UI-Indonesia/Project
From 2013.igem.org
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<br>Indonesia is one of the high burden countries for TB. The currently used method for detecting TB, the acid fast bacilli smear, has several difficulties such as getting the sputum (instead of saliva), and training the laboratory personnel to do the test. Based on this problem, UI iGEM team are trying to create a reliable, portable, and easy to use diagnostic tool for detecting TB. We are constructing a biosensor consist of highly specific antibody bound to a reporter to detect the presence of protein Ag85, a novel TB biomarker.</br> | <br>Indonesia is one of the high burden countries for TB. The currently used method for detecting TB, the acid fast bacilli smear, has several difficulties such as getting the sputum (instead of saliva), and training the laboratory personnel to do the test. Based on this problem, UI iGEM team are trying to create a reliable, portable, and easy to use diagnostic tool for detecting TB. We are constructing a biosensor consist of highly specific antibody bound to a reporter to detect the presence of protein Ag85, a novel TB biomarker.</br> | ||
<br>Here in this project, we are going to create a biosensor that will detect the presence of antigen 85, a potential tuberculosis biomarker. Antigen 85 is found in sputum, urine and most abundantly, blood serum of TB patient. Hence, blood serum of TB suspect will be used for the test. This biosensor designed to be able to detect TB more specifically than the existed method. The biosensor itself utilize split betagalactosidase combined with scFv (single chain fragment variable).</br> | <br>Here in this project, we are going to create a biosensor that will detect the presence of antigen 85, a potential tuberculosis biomarker. Antigen 85 is found in sputum, urine and most abundantly, blood serum of TB patient. Hence, blood serum of TB suspect will be used for the test. This biosensor designed to be able to detect TB more specifically than the existed method. The biosensor itself utilize split betagalactosidase combined with scFv (single chain fragment variable).</br> | ||
| - | <br>To complete the biosensor we created, characterization method to analyze the presence of Antigen 85 qualitatively and quantitatively already constructed. Characterization method is easy enough so this biosensor could be used as a good kit for TB detection in suburban area. </br> | + | <br>To complete the biosensor we created, characterization method to analyze the presence of Antigen 85 qualitatively and quantitatively already constructed. Characterization method is easy enough so this biosensor could be used as a good kit for TB detection in suburban area. </br> ---> |
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Revision as of 15:04, 15 August 2013
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