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Team:DTU-Denmark/Notebook/5 August 2013
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | ===Gels=== | ||
| + | Run 1% agarose gel on colony PCR products. | ||
| + | The samples are named: | ||
| + | *HAO 1 | ||
| + | *HAO 2 | ||
| + | *HAO 3 | ||
| + | *HAO 4 | ||
| + | *HAO 5 | ||
| + | *HAO 6 | ||
| + | *HAO 7 | ||
| + | *HAO 8 | ||
| + | Samples 1-5 and 8 were top10 transformant colonies. Colonies 1-5 were small (1mm wide) more condense and more difficult to collect; this may be due to the proteins expressed on the membrane that make it thicker. Colony 8 was wider (3mm wide) and more sticky. | ||
| + | Samples 6 and 7 are normal transformant colonies. | ||
==Results== | ==Results== | ||
Revision as of 13:34, 5 August 2013
5 August 2013
Contents |
lab 208
Main purpose
- Colony PCR for HAO
- USER reaction
Who was in the lab
Kristian, Julia, Natalia, Henrike
Procedure
Gels
Run 1% agarose gel on colony PCR products. The samples are named:
- HAO 1
- HAO 2
- HAO 3
- HAO 4
- HAO 5
- HAO 6
- HAO 7
- HAO 8
Samples 1-5 and 8 were top10 transformant colonies. Colonies 1-5 were small (1mm wide) more condense and more difficult to collect; this may be due to the proteins expressed on the membrane that make it thicker. Colony 8 was wider (3mm wide) and more sticky. Samples 6 and 7 are normal transformant colonies.
Results
1% agarose gel
- 1kb ladder
- HAO 1 (from top 10 transformants)
- HAO 2
- HAO 3
- HAO 4
- HAO 5
- HAO 6
- HAO 7
- HAO 8
- 1 kb ladder
Conclusion
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