lab 208

Main purpose

• Colony PCR for HAO
• USER reaction

Who was in the lab

Kristian, Julia, Natalia, Henrike

Procedure

Gels

Run 1% agarose gel on colony PCR products. The samples are named:

• HAO 1
• HAO 2
• HAO 3
• HAO 4
• HAO 5
• HAO 6
• HAO 7
• HAO 8

Samples 1-5 and 8 were top10 transformant colonies. Colonies 1-5 were small (1mm wide) more condense and more difficult to collect; this may be due to the proteins expressed on the membrane that make it thicker. Colony 8 was wider (3mm wide) and more sticky. Samples 6 and 7 are normal transformant colonies.

Results

1% agarose gel

• 1kb ladder
• HAO 1 (from top 10 transformants)
• HAO 2
• HAO 3
• HAO 4
• HAO 5
• HAO 6
• HAO 7
• HAO 8
• 1 kb ladder

Conclusion

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