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From 2013.igem.org

Revision as of 11:22, 6 August 2013 (view source) FelicityHeath (Talk | contribs) (→Digest) ← Older edit		Revision as of 11:43, 6 August 2013 (view source) FelicityHeath (Talk | contribs) (→Liquid cultures) Newer edit →
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-	== Liquid cultures ==
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-	We made liquid cultures of:
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-	Cph8 + RBS - K592018
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-	B0015 - Terminator
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-	K592022 - Cyan (w/RBS and promoter)
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-	K864404 - Promoter, RBS and cyan
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-	K592011 - Cyan pigment
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-	S05058
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	== Digest ==		== Digest ==

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Revision as of 11:43, 6 August 2013

Digest

We followed advice from a PhD student and did:

• Positive controls of:

- RFP cut with E and S

- RFP cut with X and P

• Negative control of:

- Just water

The buffer and BSA were vortexed before use to make sure they were completely defrosted.

We got fresh purite water each time we needed it.

We incubated at 37°C for 30 minutes and at 80°C for 20 minutes.