Team:Bielefeld-Germany/Labjournal/Molecular

From 2013.igem.org

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       <col width="50">
       <col width="50">
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<tr><td>_</td> <td>1x</td> <td>8</td> </tr>
+
<tr><td>Reagenz</td> <td>1x</td> <td>8</td> </tr>
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<td>Buffer 5x</td> <td>5,0</td> </td>44</td>
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<td>Buffer 5x</td> <td>5,0</td> <td>44</td>
MgSO4 0
MgSO4 0
dNTPs 2,5 22
dNTPs 2,5 22

Revision as of 22:40, 6 August 2013




PCR Protocols and Programs

Phusion 25 µl

MgSO4 0 dNTPs 2,5 22 Primer1 1,0 8,8 Primer2 1,0 8,8 Template 1,5 13,2 DMSO 2,0 17,6 Polymerase 0,5 4,4 H2O 11,5 101,2
Reagenz 1x 8
Buffer 5x 5,0 44