Team:UNITN-Trento/Protocols
From 2013.igem.org
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- | To test if our | + | To test if our system was able to accelerate or to slow down fruit ripening, we designed an ermetically closed jam jar with a rubber hose connector. These jars contained our test-fruit and each one was connected to a flask. The flasks contained 300 ml of induced (or not) culture when his O.D.600 reached 0.8. The flasks contained also a stirrer. The cultures were maintained at 37 °C using a laboratory heating plate connected to a digital thermometer immersed in the culture.<br/> |
- | + | For six days, every morning the culture in the flasks was substituted with a new induced (or not) colture .<br/> | |
- | + | Furthermore, canonical jam jars (i.e.: with no connector) were adopted to contain the negative control fruit samples.<br/> | |
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Revision as of 09:36, 7 August 2013