Team:Hong Kong HKU/surface decoration

From 2013.igem.org

(Difference between revisions)
(Created page with "<html> <head> <meta charset="utf-8"> <title>E. capsi</title> <style type="text/css"> body,td,th { font-family: "Gill Sans", "Gill Sans MT", "Myriad Pro", "DejaVu Sans Conden...")
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{{:Team:Hong_Kong_HKU/templates/header}}
<html>
<html>
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<head>
 
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<meta charset="utf-8">
 
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<title>E. capsi</title>
 
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<style type="text/css">
 
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body,td,th {
 
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font-family: "Gill Sans", "Gill Sans MT", "Myriad Pro", "DejaVu Sans Condensed", Helvetica, Arial, sans-serif;
 
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font-style: normal;
 
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}
 
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body {
 
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background-image: url("https://static.igem.org/mediawiki/2013/0/02/Hku_background.jpg");
 
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background-repeat
 
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}
 
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</style>
 
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</head>
 
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<body>
<body>
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<div id="divWrapper">
 
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<div id="divHeader"><img src="https://static.igem.org/mediawiki/2013/c/cb/Hku_logo.jpg" width="960" height="240"/></div>
 
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    <div id="divlinks">
 
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    <div id="divNav">
 
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        <style type="text/css">
 
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.tab{
 
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font-family: arial, verdana, san-serif;
 
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font-size: 14px;
 
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}
 
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.asd
 
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{
 
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text-decoration: none;
 
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font-family: arial, verdana, san-serif;
 
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font-size: 13px;
 
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color:00CC66;
 
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}
 
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/*****remove the list style****/
 
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#nav {
 
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margin:0;
 
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padding:0;
 
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list-style:none;
 
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}
 
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/*****LI display inline *****/
 
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#nav li {
 
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float:left;
 
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display:block;
 
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width:190px;
 
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background:#00661A;
 
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position:relative;
 
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z-index:500;
 
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margin:0 1px;
 
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}
 
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@charset "utf-8";
 
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body, h1, h2, h3, h4, h5, h6, p, div, ul , li{
 
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margin:0;
 
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padding:0;
 
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}
 
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body {
 
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font-family:Segoe, "Segoe UI", "DejaVu Sans", "Trebuchet MS", Verdana, sans-serif;
 
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}
 
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#divWrapper{
 
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width:960px;
 
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margin:0 auto;
 
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}
 
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#divHeader{
 
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width:960px;
 
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margin:0 auto;
 
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text-align:center;
 
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}
 
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#divHeader img{
 
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padding-top:20px;
 
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padding-bottom:5px;
 
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}
 
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#divline {
 
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width:960px;
 
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background:#F63;
 
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height:2px;
 
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}
 
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#divlinks{
 
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width:960px;
 
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background:#666;
 
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}
 
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#divNav{
 
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width:960px;
 
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background:#000;
 
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height:32px;
 
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margin:0 auto;
 
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}
 
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#divAbout {
 
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width:960px;
 
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background:#000;
 
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margin:0 auto;
 
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}
 
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#divContent{
 
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width:960px;
 
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height:1000px
 
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margin:0 auto;
 
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border:1px auto solid #000;
 
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text-align:center;
 
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}
 
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#divContent img{
 
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float:left;
 
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border:1px solid #000;
 
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background #333;
 
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padding:16px;
 
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margin:0.7em;
 
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background:-moz-linear-gradient(top,#666,#333);
 
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background:-webkit-gradient(linear,left,top, left bottom, from (#666), to(#333));
 
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}
 
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/*****parent menu*****/
 
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#nav li a {
 
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display:block;
 
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padding:8px 5px 0 5px;
 
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font-weight:700;
 
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height:23px;
 
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text-decoration:none;
 
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color:#00994D;
 
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text-align:center;
 
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color:#FFCC33;
 
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}
 
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#nav li a:hover
 
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{
 
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color:#FFFFFF;
 
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}
 
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/* style for default selected value */ #nav a.selected {
 
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color:00CC66;
 
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}
 
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/* submenu */ #nav ul
 
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{
 
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position:absolute;
 
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left:0;
 
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display:none;
 
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margin:0 0 0 -1px;
 
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padding:0;
 
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list-style:none;
 
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}
 
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#nav ul li
 
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{
 
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width:190px;
 
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float:left;
 
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border-top:1px solid #fff;
 
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}
 
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/* display block will make the link fill the whole area of LI */ #nav ul a
 
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{
 
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display:block;
 
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height:23px;
 
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padding: 8px 5px;
 
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color:#FFFFF
 
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}
 
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#nav ul a:hover
 
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{
 
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text-decoration:underline;
 
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padding-left:15px;
 
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}
 
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</style>
 
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<script type="text/javascript">
 
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ret=jQuery.merge(ret,elem);});return ret;},attr:function(elem,name,value){if(!elem||elem.nodeType==3||elem.nodeType==8)return undefined;var notxml=!jQuery.isXMLDoc(elem),set=value!==undefined,msie=jQuery.browser.msie;name=notxml&&jQuery.props[name]||name;if(elem.tagName){var special=/href|src|style/.test(name);if(name=="selected"&&jQuery.browser.safari)elem.parentNode.selectedIndex;if(name in elem&&notxml&&!special){if(set){if(name=="type"&&jQuery.nodeName(elem,"input")&&elem.parentNode)throw"type property can't be changed";elem[name]=value;}if(jQuery.nodeName(elem,"form")&&elem.getAttributeNode(name))return elem.getAttributeNode(name).nodeValue;return elem[name];}if(msie&&notxml&&name=="style")return jQuery.attr(elem.style,"cssText",value);if(set)elem.setAttribute(name,""+value);var attr=msie&&notxml&&special?elem.getAttribute(name,2):elem.getAttribute(name);return attr===null?undefined:attr;}if(msie&&name=="opacity"){if(set){elem.zoom=1;elem.filter=(elem.filter||"").replace(/alpha\([^)]*\)/,"")+(parseInt(value)+''=="NaN"?"":"alpha(opacity="+value*100+")");}return elem.filter&&elem.filter.indexOf("opacity=")>=0?(parseFloat(elem.filter.match(/opacity=([^)]*)/)[1])/100)+'':"";}name=name.replace(/-([a-z])/ig,function(all,letter){return letter.toUpperCase();});if(set)elem[name]=value;return elem[name];},trim:function(text){return(text||"").replace(/^\s+|\s+$/g,"");},makeArray:function(array){var ret=[];if(array!=null){var i=array.length;if(i==null||array.split||array.setInterval||array.call)ret[0]=array;else
 
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</script>
 
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<script type="text/javascript">
 
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$(document).ready(function () {
 
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$('#nav li').hover(
 
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function () {
 
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//show its submenu
 
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$('ul', this).slideDown(350);
 
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},
 
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function () {
 
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//hide its submenu
 
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$('ul', this).slideUp(350);
 
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);
 
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<input type=hidden name=arav value="5*#*#*3*#*#*2*#*#*1*#*#*3*#*#*2"><ul id='nav'>
 
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<li> <a href='#'>E. Capsid</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU">At a Glance</a></li>
 
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<li style='background-color:#454141;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/surface_decoration">Surface Decoration</a></li>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/ppk">PPK</a></li>
 
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</ul>
 
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<li> <a href='#'>Human Practice</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/safety">Safety</a></li>
 
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<li style='background-color:#454141;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/induction_day">Induction Day</a></li>
 
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</ul>
 
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<li> <a href='#'>Modelling</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/report">Report</a></li>
 
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</ul>
 
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<li> <a href='#'>Extras</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/protocols">Protocols</a></li>
 
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<li style='background-color:#454141;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/collaboration">Collaboration</a></li>
 
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<li style='background-color:#000;'><a href"https://2013.igem.org/Team:Hong_Kong_HKU/diary"=>Diary</a></li>
 
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</ul>
 
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<li> <a href='#'>Team</a>
 
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<ul>
 
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<li style='background-color:#000;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/people">People</a></li>
 
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<li style='background-color:#454141;'><a href="https://2013.igem.org/Team:Hong_Kong_HKU/contact_us">Contact us</a></li>
 
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</ul>
 
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</li>
 
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Revision as of 07:16, 20 August 2013



There are 5 Eut Shell protein genes (S, M, N, L, K). EutS and EutM form the edges and facets of the shell. EutN cap the vertexes of the capsid. EutL facilitates fated transport into and out of the BMC. EutK’s role is at present unclear. Among all Eut Shell proteins, crystallographic data indicates the N-terminal of EutS are found on the exterior surface of the Eut MCP, making it our possible candidates for tag peptide fusion. Other 4 shell proteins bury both termini deeply and not suitable for peptide fusion. To our surprise, a study in 2012 showed that EutS alone is necessary and sufficient for the formation of shells in vivo. Hence, homogenous EutS shell proteins might offer a simplier strategy for engineering of cell-specific targeting capsid, and potentially having higher specificity and affinity. Hence, we will construct and compare two types of MCPs: (1) Single expression of homogenous Tagged EutS (2) Co-expression of Heterogenous Taggest EutS and EutMNLK