Team:HokkaidoU Japan

From 2013.igem.org

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Recently, more and more projects for iGEM involve precise dynamic system or complex chemical compounds. These projects need minute regulation of gene expressions. However, few BioBrick parts controlling gene expression such as promoters or RBS’s are reliable, and many iGEMers choose one among these parts too carelessly.
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<p>This year, we make well-selected sets of promoters and RBS’s with wide variation of strength. These parts are evaluated strictly. We mutate a certain region of a basic part to make these sets, so their performances are reliable.</p>
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In the field of synthetic biology, a lot of precise and accurate dynamic systems have been designed. For the construction of these systems, (1) transparency of their structures, (2) reliability of their functions, (3) reproducibility of expected results when they are used and (4) safety of them are crucial. Therefore, as for BioBricks which support these systems, especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable by their nucleotide sequences.
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<p>Furthermore, we make the Optimization Kit which enables you to select the most suitable parts for your project easily. This Kit will help other iGEMers create more complex and effective devises.</p>
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<p>Our project this year is to make the libraries of promoters and RBS’s which are made by randomization of their certain regions. These parts are evaluated strictly and have high reproducibility. Furthermore, we will make the“Promoter Tuning Kit” and the “RBS Tuning Kit”, which enables you to easily select the most suitable parts when you design a biological system. These Kits will contribute to improve efficiency of the construction of more complex systems drastically.</p>
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<p>Besides, we give a presentation at our university and some high schools about iGEM and genetics. On that occasion we get to know how the citizens think about gene recombination by means of questionnaires and try to let them have deeper thought about it through our communication. We hope these activities will lead the society where people accept genetic engineering technology. </p>
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<p>In Human Practice, we are planning to give a presentation about genetics and the activity of iGEM through our Universtiy Festival and visit some high schools. We are expecting to learn how the younger students think about genetic recombination by means of reading answers to our questionnaires and trying to help their understanding of it through communication. We hope these activities will lead the society where younger people accept genetic engineering.
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|[[Image:HokkaidoU_Japan_team.png|right|frame|Your team picture]]
|[[Image:HokkaidoU_Japan_team.png|right|frame|Your team picture]]

Revision as of 13:58, 9 August 2013

HOKKAIDO U

Maestro E.coli

Motivation


Description

In the field of synthetic biology, a lot of precise and accurate dynamic systems have been designed. For the construction of these systems, (1) transparency of their structures, (2) reliability of their functions, (3) reproducibility of expected results when they are used and (4) safety of them are crucial. Therefore, as for BioBricks which support these systems, especially parts controlling gene expression such as promoters or RBSs, it is desired that their prospective functions are explainable by their nucleotide sequences.

Our project this year is to make the libraries of promoters and RBS’s which are made by randomization of their certain regions. These parts are evaluated strictly and have high reproducibility. Furthermore, we will make the“Promoter Tuning Kit” and the “RBS Tuning Kit”, which enables you to easily select the most suitable parts when you design a biological system. These Kits will contribute to improve efficiency of the construction of more complex systems drastically.

In Human Practice, we are planning to give a presentation about genetics and the activity of iGEM through our Universtiy Festival and visit some high schools. We are expecting to learn how the younger students think about genetic recombination by means of reading answers to our questionnaires and trying to help their understanding of it through communication. We hope these activities will lead the society where younger people accept genetic engineering.


Team HokkaidoU_Japan


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