Team:Fudan/Project

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== '''Overall project''' ==
 
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Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)
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== A Novel Aminoglycoside Detection Strategy==
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How do you deal with the petri dishes after the experiment? Throwing them away? It seems to happen in most of the labs. And after the simply discarding the litter, garbage will finally go into the environment. While the nutrients are broken down by decomposers, the antibiotics will stay there.
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This summer, we focus on the detection of a group of antibiotics called aminoglycosides (for example the Kanamycin we used as a selection marker). The problem of aminoglycosides are more serious because compared with other kinds of antibiotics, they are more difficult to be break down and tested, and they are most commonly used in the research, medical treatment, industry and agriculture. Even under the high temperature they are not easy to be inactivated. Moreover, they will maintain in the blood for weeks, much more than other kinds of antibiotics such as penicillin. The accumulation of aminoglycoside will also lead to ototoxicity and nephrotoxicity.
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The problem is more serious in the industrial and agricultural productions. For example, in a life of dairy cow, the antibiotics are everywhere. What it eats can be forage addicted with certain kinds of antibiotics, and sometimes even the antibiotic-producing bacteria. When it gets sickness, the veterinary medicine usually includes the antibiotics as well. These chemicals will also exist in the milk for days to weeks.
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Though there are provisions in many countries on these issues, for example, for the typical milk discard period after a case of clinical mastitis including the treatment and the withdrawal time, the abuse of antibiotics is still common. Research has shown each year deaths related to antibiotic abuse total 80,000 on the Chinese mainland and 80 billion yuan (13 billion dollars) of the medical budget.’(China Daily 2012.07.06 Crackdown on Antibiotic Abuse).
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By studying the regulation of resistance, we have found the natural parts which are sensitive to the antibiotics. With the quantitative methods, we are measuring the response curve of them. Then the optimized devices will be constructed for aminoglycosides detection.
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Both the environment and people health are threatened. The antibiotics in the environment and animals will finally accumulate in the human body, then lead to the chronic toxicity and harm to the normal flora of humans. Therefore, obviously better antibiotics detection methods are demanded.
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In our case, we focus on the detection of a group of antibiotics called aminoglycosides (for example the Kanamycin we used as a selection marker). The problem of aminoglycosides are more serious because compared with other kinds of antibiotics, they are more difficult to be break down and tested, and they are most commonly used in the research, medical treatment, industry and agriculture. Even under the high temperature they are not easy to be inactivated. Moreover, they will maintain in the blood for weeks, much more than other kinds of antibiotics such as penicillin. The accumulation of aminoglycoside will also lead to ototoxicity and nephrotoxicity.  
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Meanwhile, we are also studying on the molecular dynamics and mechanisms of regulation of resistance. By modifying and estimating one riboswitch conserved in plenty of aminoglycoside acetyl transferases and aminoglycoside adenyl transferases, we are trying to find a novel principle to design and develop the bicistron-based translation initiation elements which can function more precisely and reliably.
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By studying the regulation of resistance, we find the natural parts which are sensitive to the antibiotics. With the quantitative methods, we are measuring the response curve. Then optimized devices will be constructed for aminoglycosides detection.
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Meanwhile, we are also studying on the molecular dynamics and mechanisms of regulation of resistance. By modifying and estimating one riboswitch conserved in plenty of aminoglycoside acetyl transferases and aminoglycoside adenyl transferases, we are trying to find a novel principle to design and develop the aptamer-based translation initiation elements which can function more precisely and reliably.
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== Project Details==
== Project Details==

Revision as of 03:56, 9 August 2013


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Contents

A Novel Aminoglycoside Detection Strategy

This summer, we focus on the detection of a group of antibiotics called aminoglycosides (for example the Kanamycin we used as a selection marker). The problem of aminoglycosides are more serious because compared with other kinds of antibiotics, they are more difficult to be break down and tested, and they are most commonly used in the research, medical treatment, industry and agriculture. Even under the high temperature they are not easy to be inactivated. Moreover, they will maintain in the blood for weeks, much more than other kinds of antibiotics such as penicillin. The accumulation of aminoglycoside will also lead to ototoxicity and nephrotoxicity.

By studying the regulation of resistance, we have found the natural parts which are sensitive to the antibiotics. With the quantitative methods, we are measuring the response curve of them. Then the optimized devices will be constructed for aminoglycosides detection.

Meanwhile, we are also studying on the molecular dynamics and mechanisms of regulation of resistance. By modifying and estimating one riboswitch conserved in plenty of aminoglycoside acetyl transferases and aminoglycoside adenyl transferases, we are trying to find a novel principle to design and develop the bicistron-based translation initiation elements which can function more precisely and reliably.

Project Details

Part 2

The Experiments

Part 3

Results

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